XRCC3 Recombinant Rabbit Monoclonal Antibody [JE38-43]
Catalog# HA722399
XRCC3 Recombinant Rabbit Monoclonal Antibody [JE38-43]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
XRCC3 Recombinant Rabbit Monoclonal Antibody [JE38-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human XRCC3 aa 247-346 / 346.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
NCI-H1299 cell lysate, MDA-MB-468 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate.
Conjugation
unconjugated
Clone Number
JE38-43
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
DNA repair protein XRCC3 is a protein that in humans is encoded by the XRCC3 gene. This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. The XRCC3 protein is one of five paralogs of RAD51, including RAD51B (RAD51L1), RAD51C (RAD51L2), RAD51D (RAD51L3), XRCC2 and XRCC3. They each share about 25% amino acid sequence identity with RAD51 and each other.
Background References
1. Longo MA et al. RAD51C-XRCC3 structure and cancer patient mutations define DNA replication roles. Nat Commun. 2023 Jul
2. Hu S et al. Association between XRCC3 rs861539 Polymorphism and the Risk of Ovarian Cancer: Meta-Analysis and Trial Sequential Analysis. Biomed Res Int. 2022 Aug
Subcellular Location
Nucleus, Cytoplasm, perinuclear region, Mitochondrion.
Synonyms
CMM6 antibody
DNA repair protein XRCC3 antibody
RAD51 like antibody
X ray repair complementing defective repair in Chinese hamster antibody
X ray repair complementing defective repair in Chinese hamster cells 3 antibody
X ray repair cross complementing protein 3 antibody
X-ray repair cross-complementing protein 3 antibody
XRCC 3 antibody
XRCC3 antibody
XRCC3_HUMAN antibody
Images
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Western blot analysis of XRCC3 on different lysates with Rabbit anti-XRCC3 antibody (HA722399) at 1/1,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: MDA-MB-468 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 45 kDa
Exposure time: 2 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722399) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"