KGF Recombinant Rabbit Monoclonal Antibody [JE40-14]
Catalog# HA722404
KGF Recombinant Rabbit Monoclonal Antibody [JE40-14]
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WB
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
KGF Recombinant Rabbit Monoclonal Antibody [JE40-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human KGF aa 1-100 / 194.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB
Molecular Weight
Predicted band size: 23 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE40-14
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Background References
1. Liu X et al. FGF7-induced E11 facilitates cell-cell communication through connexin43. Int J Biol Sci. 2021 Sep
2. Milton CI et al. FGF7-FGFR2 autocrine signaling increases growth and chemoresistance of fusion-positive rhabdomyosarcomas. Mol Oncol. 2022 Mar
Subcellular Location
Secreted.
Synonyms
FGF 7 antibody
FGF-7 antibody
Fgf7 antibody
FGF7_HUMAN antibody
Fibroblast growth factor 7 antibody
HBGF 7 antibody
HBGF-7 antibody
HBGF7 antibody
Heparin binding growth factor 7 antibody
Heparin-binding growth factor 7 antibody
ExpandImages
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Western blot analysis of KGF on different lysates with Rabbit anti-KGF antibody (HA722404) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 28 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722404) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"