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Integrin alpha 9 Recombinant Rabbit Monoclonal Antibody [JE41-58]

Usd: 385

Specification

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Catalog# HA722406

Integrin alpha 9 Recombinant Rabbit Monoclonal Antibody [JE41-58]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Integrin alpha 9 Recombinant Rabbit Monoclonal Antibody [JE41-58]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Integrin alpha 9 aa 50-150.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 114 kDa

Positive Control

HepG2 cell lysate, A549 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

JE41-58

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Integrin alpha-9 is a protein that in humans is encoded by the ITGA9 gene. This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for tenascin-C, VCAM1 and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers.

Background References

1. Hou H et al. ITGA9 Inhibits Proliferation and Migration of Dermal Microvascular Endothelial Cells in Psoriasis. Clin Cosmet Investig Dermatol. 2022 Dec

2. Jin S et al. Depletion of CUL4B in macrophages ameliorates diabetic kidney disease via miR-194-5p/ITGA9 axis. Cell Rep. 2023 Jun

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q13797 Human

Entrez Gene: 104099 Mouse

Entrez Gene: 685004 Rat

Synonyms

Alpha RLC antibody

Integrin alpha 4 like antibody

Integrin alpha RLC antibody

Integrin alpha-9 antibody

Integrin alpha-RLC antibody

ITA9_HUMAN antibody

ITGA 4L antibody

ITGA 9 antibody

ITGA4L antibody

ITGA9 antibody

Expand

Images

  • Western blot analysis of Integrin alpha 9 on different lysates with Rabbit anti-Integrin alpha 9 antibody (<a href="/products/HA722406" style="font-weight: bold;text-decoration: underline;">HA722406</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: A549 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 114 kDa<br />Observed band size: 114 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722406" style="font-weight: bold;text-decoration: underline;">HA722406</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Integrin alpha 9 on different lysates with Rabbit anti-Integrin alpha 9 antibody (HA722406) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: A549 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722406) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"