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COX6B1 Recombinant Rabbit Monoclonal Antibody [JE59-16]

Usd: 385

Specification

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Catalog# HA722407

COX6B1 Recombinant Rabbit Monoclonal Antibody [JE59-16]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

COX6B1 Recombinant Rabbit Monoclonal Antibody [JE59-16]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human COX6B1 aa 37-86 / 86.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 10 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, Caco-2 cell lysate, human colon tissue.

Conjugation

unconjugated

Clone Number

JE59-16

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:8,000

  • IF-Tissue

  • 1:1,000-1:4,000

Target

Function

Cytochrome c oxidase subunit 6B1 is an enzyme that in humans is encoded by the COX6B1 gene. Cytochrome c oxidase 6B1 is a subunit of the cytochrome c oxidase complex, also known as Complex IV, the last enzyme in the mitochondrial electron transport chain. Mutations of the COX6B1 gene are associated with severe infantile encephalomyopathy and mitochondrial complex IV deficiency (MT-C4D).

Background References

1. Chen L et al. [miR-30b-3p Inhibits the Proliferation and Invasion of Lung Adenocarcinoma by Targeting COX6B1]. Zhongguo Fei Ai Za Zhi. 2022 Aug

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: P14854 Human

Synonyms

COX 6B antibody

COX VIb 1 antibody

COX VIb-1 antibody

COX6B antibody

COX6B1 antibody

COXG antibody

COXVIb1 antibody

CX6B1_HUMAN antibody

Cytochrome c oxidase subunit 6B1 antibody

Cytochrome c oxidase subunit VIb antibody

Expand

Images

  • Western blot analysis of COX6B1 on different lysates with Rabbit anti-COX6B1 antibody (<a href="/products/HA722407" style="font-weight: bold;text-decoration: underline;">HA722407</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Caco-2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 10 kDa<br />Observed band size: 10 kDa<br /><br />Exposure time: 18 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722407" style="font-weight: bold;text-decoration: underline;">HA722407</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of COX6B1 on different lysates with Rabbit anti-COX6B1 antibody (HA722407) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Caco-2 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

    Exposure time: 18 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722407) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-COX6B1 antibody (<a href="/products/HA722407" style="font-weight: bold;text-decoration: underline;">HA722407</a>) at 1/8,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722407" style="font-weight: bold;text-decoration: underline;">HA722407</a>) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-COX6B1 antibody (HA722407) at 1/8,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722407) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"