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Cadherin 16 Recombinant Rabbit Monoclonal Antibody [JE60-37]

Usd: 385

Specification

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Catalog# HA722408

Cadherin 16 Recombinant Rabbit Monoclonal Antibody [JE60-37]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Cadherin 16 Recombinant Rabbit Monoclonal Antibody [JE60-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cadherin 16 aa 1-200 / 829.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 90 kDa

Positive Control

Mouse kidney tissue lysates, human kidney tissue, mouse kidney tissue.

Conjugation

unconjugated

Clone Number

JE60-37

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

Cadherin-16 is a protein that in humans is encoded by the CDH16 gene. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in the kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development.

Background References

1. Lennartz M et al. Reduced CDH16 expression is linked to poor prognosis in clear cell renal cell carcinoma 16. Urol Oncol. 2022 Jul

2. de Cristofaro T et al. An essential role for Pax8 in the transcriptional regulation of cadherin-16 in thyroid cells. Mol Endocrinol. 2012 Jan

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: O75309 Human

SwissProt: O88338 Mouse

Synonyms

CAD16_HUMAN antibody

Cadherin 16 antibody

Cadherin 16 KSP cadherin antibody

Cadherin kidney antibody

Cadherin-16 antibody

CDH 16 antibody

CDH16 antibody

Kidney cadherin antibody

Kidney specific cadherin antibody

Kidney-specific cadherin antibody

Expand

Images

  • Western blot analysis of Cadherin 16 on mouse kidney tissue lysates with Rabbit anti-Cadherin 16 antibody (<a href="/products/HA722408" style="font-weight: bold;text-decoration: underline;">HA722408</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 90 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 8 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722408" style="font-weight: bold;text-decoration: underline;">HA722408</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cadherin 16 on mouse kidney tissue lysates with Rabbit anti-Cadherin 16 antibody (HA722408) at 1/1,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 90 kDa
    Observed band size: 130 kDa

    Exposure time: 8 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cadherin 16 antibody (<a href="/products/HA722408" style="font-weight: bold;text-decoration: underline;">HA722408</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722408" style="font-weight: bold;text-decoration: underline;">HA722408</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cadherin 16 antibody (HA722408) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722408) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cadherin 16 antibody (<a href="/products/HA722408" style="font-weight: bold;text-decoration: underline;">HA722408</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722408" style="font-weight: bold;text-decoration: underline;">HA722408</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Cadherin 16 antibody (HA722408) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722408) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"