MSRB3 Recombinant Rabbit Monoclonal Antibody [JE64-83]
Catalog# HA722410
MSRB3 Recombinant Rabbit Monoclonal Antibody [JE64-83]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MSRB3 Recombinant Rabbit Monoclonal Antibody [JE64-83]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human MSRB3 aa 1-200 / 192.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 21 kDa
Positive Control
A549 cell lysate, HUVEC cell lysate, U-2 OS cell lysate, HeLa cell lysate, mouse testis tissue lysate, mouse heart tissue lysate, rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-83
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
The methionine sulfoxide reductase B3 gene (MsrB3), a protein repair enzyme, has been implicated in large scale stereocilia bundle degeneration, as well as many other factors such as gestational age and tolerance to cold environments in plants. Although the exact process of pathogenesis is unknown, it seems to be related to apoptotic cell death. A study based on splicing morpholinos to down-regulate MsrB3 expression in zebrafish showed shorter, thinner, and more crowded cilia, as well as small, misplaced otoliths. Several stereocilia also underwent apoptosis. Injection with wild-type MsrB3 mRNA rescued auditory deficits, suggesting MsrB3 helps prevent apoptosis.
Background References
1. Ye X et al. MSRB3 promotes the progression of clear cell renal cell carcinoma via regulating endoplasmic reticulum stress. Pathol Res Pract. 2020 Feb
2. Paris JM et al. Ear type in sheep is associated with the MSRB3 locus. Anim Genet. 2020 Dec
Subcellular Location
Endoplasmic reticulum, Mitochondrion.
Synonyms
Deafness, Autosomal Recessive 74 antibody
DFNB74 antibody
FLJ36866 antibody
Methionine R sulfoxide reductase B mitochondrial antibody
Methionine sulfoxide reductase B3 antibody
Methionine-R-sulfoxide reductase B3 antibody
MsrB3 antibody
MSRB3_HUMAN antibody
Images
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Western blot analysis of MSRB3 on different lysates with Rabbit anti-MSRB3 antibody (HA722410) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HUVEC cell lysate (20 µg/Lane)
Lane 3: U-2 OS cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (30 µg/Lane)
Lane 6: Mouse heart tissue lysate (30 µg/Lane)
Lane 7: Rat testis tissue lysate (30 µg/Lane)
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722410) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"