PWP1 Recombinant Rabbit Monoclonal Antibody [JE65-28]
Catalog# HA722412
PWP1 Recombinant Rabbit Monoclonal Antibody [JE65-28]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PWP1 Recombinant Rabbit Monoclonal Antibody [JE65-28]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PWP1 aa 452-501 / 501.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, C6 cell lysate.
Conjugation
unconjugated
Clone Number
JE65-28
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Periodic tryptophan protein 1 homolog is a protein that in humans is encoded by the PWP1 gene. The protein encoded by this gene contains several WD-40 repeats and is found mostly in the nucleus. The expression and localization of this protein are cell cycle dependent. Expression of this gene is upregulated in pancreatic adenocarcinoma. Three transcript variants encoding different isoforms have been found for this gene.
Background References
1. Wei L et al. PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin. Onco Targets Ther. 2020 Oct
2. Yu Y et al. Pwp1 regulates telomere length by stabilizing shelterin complex and maintaining histone H4K20 trimethylation. Cell Discov. 2019 Nov
Subcellular Location
Nucleus, nucleolus, Chromosome.
Synonyms
Keratinocyte protein IEF SSP 9502 antibody
Periodic tryptophan protein 1 homolog antibody
PWP1 antibody
PWP1 homolog antibody
PWP1_HUMAN antibody
Images
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Western blot analysis of PWP1 on different lysates with Rabbit anti-PWP1 antibody (HA722412) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 70 kDa
Exposure time: Lane 1-2: 25 seconds; Lane 3-4: 1 minute 6 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722412) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"