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HSPA12A Recombinant Rabbit Monoclonal Antibody [JE01-78]

Usd: 385

Specification

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Catalog# HA722425

HSPA12A Recombinant Rabbit Monoclonal Antibody [JE01-78]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HSPA12A Recombinant Rabbit Monoclonal Antibody [JE01-78]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HSPA12A aa 101-150 / 675.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Target Molecular Weight

Predicted band size: 75 kDa

Positive Control

HEK-293 cell lysate, U-87 MG cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JE01-78

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:500

  • IP

  • 1-2μg/sample

Target

Function

Heat shock 70kDa protein 12A also known as HSPA12A is a human gene. The protein encoded by this gene is a member of the Hsp70 family of heat shock proteins. Predicted to enable ATP binding activity.

Background References

1. Du S et al. Hepatocyte HSPA12A inhibits macrophage chemotaxis and activation to attenuate liver ischemia/reperfusion injury via suppressing glycolysis-mediated HMGB1 lactylation and secretion of hepatocytes. Theranostics. 2023 Jul

2. Wang J et al. HSPA12A controls cerebral lactate homeostasis to maintain hippocampal neurogenesis and mood stabilization. Transl Psychiatry. 2023 Aug

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: O43301 Human

SwissProt: Q8K0U4 Mouse

Entrez Gene: 307997 Rat

Synonyms

FLJ13874 antibody

Heat shock 70 kDa protein 12A antibody

heat shock 70kD protein 12A antibody

heat shock 70kDa protein 12A antibody

HS12A_HUMAN antibody

Hspa12a antibody

KIAA0417 antibody

Images

  • Western blot analysis of HSPA12A on different lysates with Rabbit anti-HSPA12A antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (10 µg/Lane)<br />Lane 2: U-87 MG cell lysate (10 µg/Lane)<br />Lane 3: Human brain tissue lysate (20 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 5: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 75 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 1 minute 34 seconds; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HSPA12A on different lysates with Rabbit anti-HSPA12A antibody (HA722425) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (10 µg/Lane)
    Lane 2: U-87 MG cell lysate (10 µg/Lane)
    Lane 3: Human brain tissue lysate (20 µg/Lane)
    Lane 4: Mouse brain tissue lysate (20 µg/Lane)
    Lane 5: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 75 kDa
    Observed band size: 75 kDa

    Exposure time: 1 minute 34 seconds; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722425) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HSPA12A antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HSPA12A antibody (HA722425) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722425) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HSPA12A antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-HSPA12A antibody (HA722425) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722425) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HSPA12A antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HSPA12A antibody (HA722425) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722425) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • HSPA12A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with <a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/025-100-005" style="font-weight: bold;text-decoration: underline;">025-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Mouse brain tissue lysate (input)<br />Lane 2: <a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a> IP in mouse brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722425" style="font-weight: bold;text-decoration: underline;">HA722425</a> in mouse brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    HSPA12A was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA722425 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722425 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Mouse brain tissue lysate (input)
    Lane 2: HA722425 IP in mouse brain tissue lysate
    Lane 3: Rabbit IgG instead of HA722425 in mouse brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"