MRC2 Recombinant Rabbit Monoclonal Antibody [JE34-76]
Catalog# HA722429
MRC2 Recombinant Rabbit Monoclonal Antibody [JE34-76]
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WB
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Human
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unconjugated
Overview
Product Name
MRC2 Recombinant Rabbit Monoclonal Antibody [JE34-76]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human MRC2 aa 101-250 / 1,479.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 167 kDa
Positive Control
HEK-293 cell lysate, Saos-2 cell lysate.
Conjugation
unconjugated
Clone Number
JE34-76
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). The receptor recognises terminal mannose, N-acetylglucosamine and fucose residues on glycans attached to proteins found on the surface of some microorganisms, playing a role in both the innate and adaptive immune systems. Additional functions include clearance of glycoproteins from circulation, including sulphated glycoprotein hormones and glycoproteins released in response to pathological events. The mannose receptor recycles continuously between the plasma membrane and endosomal compartments in a clathrin-dependent manner.
Background References
1. Li L et al. MRC2 Promotes Proliferation and Inhibits Apoptosis of Diabetic Nephropathy. Anal Cell Pathol (Amst). 2021 Apr
2. Evans RJ et al. Endo180 (MRC2) Antibody-Drug Conjugate for the Treatment of Sarcoma. Mol Cancer Ther. 2023 Feb
Subcellular Location
Membrane.
UNIPROT
Synonyms
C-type lectin domain family 13 member E antibody
C-type mannose receptor 2 antibody
CD 280 antibody
CD280 antibody
CD280 antigen antibody
CLEC 13E antibody
CLEC13E antibody
ENDO 180 antibody
ENDO180 antibody
Endocytic receptor (macrophage mannose receptor family) antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of MRC2 on different lysates with Rabbit anti-MRC2 antibody (HA722429) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 cell lysate treated with deglycosylation
Lane 3: Saos-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 167 kDa
Observed band size: 167-250 kDa
Exposure time: Lane 1-2: 3 minutes; Lane 3: 4 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722429) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"