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Tin2 Recombinant Rabbit Monoclonal Antibody [JE35-82]

Usd: 385

Specification

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Catalog# HA722432

Tin2 Recombinant Rabbit Monoclonal Antibody [JE35-82]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Tin2 Recombinant Rabbit Monoclonal Antibody [JE35-82]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Tin2 aa 151-199 / 451.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 38 kDa

Positive Control

MCF7 cell lysate, HUVEC cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

JE35-82

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

TERF1-interacting nuclear factor 2 is a protein that in humans is encoded by the TINF2 gene. TINF2 is a component of the shelterin protein complex found at the end of telomeres.

Background References

1. Yin S et al. TIN2 deficiency leads to ALT-associated phenotypes and differentiation defects in embryonic stem cells. Stem Cell Reports. 2022 May

2. Kaur P et al. TIN2 is an architectural protein that facilitates TRF2-mediated trans- and cis-interactions on telomeric DNA. Nucleic Acids Res. 2021 Dec

Subcellular Location

Nucleus, Chromosome, telomere; Nucleus matrix.

UNIPROT

SwissProt: Q9BSI4 Human

SwissProt: Q9QXG9 Mouse

Entrez Gene: 290232 Rat

Synonyms

AW552114 antibody

D14Wsu146e antibody

DKCA3 antibody

MGC94711 antibody

TERF 1 (TRF 1) interacting nuclear factor 2 antibody

TERF 1 interacting nuclear factor 2 antibody

TERF1 (TRF1) interacting nuclear factor 2 antibody

TERF1 interacting nuclear factor 2 antibody

TERF1-interacting nuclear factor 2 antibody

Tin 2 antibody

Expand

Images

  • Western blot analysis of Tin2 on different lysates with Rabbit anti-Tin2 antibody (<a href="/products/HA722432" style="font-weight: bold;text-decoration: underline;">HA722432</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate<br />Lane 2: HUVEC cell lysate<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 1 minute 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722432" style="font-weight: bold;text-decoration: underline;">HA722432</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Tin2 on different lysates with Rabbit anti-Tin2 antibody (HA722432) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate
    Lane 2: HUVEC cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 45 kDa

    Exposure time: 1 minute 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722432) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"