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Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74]

Usd: 385

Specification

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Catalog# HA722441

Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74]

Application

  • WB

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Steroid sulfatase Recombinant Rabbit Monoclonal Antibody [PSH05-74]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Steroid sulfatase aa 22-184 and aa 235-583.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 65 kDa

Positive Control

Jurkat cell lysate HepG2 cell lysate, MCF7 cell lysate, Huh7 cell lysate, T-47D cell lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

PSH05-74

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
Tested Tested
Not Recommended
Not Recommended
mouse
Not Recommended
Not Recommended
 
 
rat
Not Recommended
Not Recommended
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:3,000

Target

Function

Catalyzes the conversion of sulfated steroid precursors, such as dehydroepiandrosterone sulfate (DHEA-S) and estrone sulfate to the free steroid. Defects in STS are the cause of ichthyosis X-linked (IXL) [MIM:308100]. Ichthyosis X-linked is a keratinization disorder manifesting with mild erythroderma and generalized exfoliation of the skin within a few weeks after birth. Affected boys later develop large, polygonal, dark brown scales, especially on the neck, extremities, trunk, and buttocks.

Background References

1. Oyama N., Satoh M., Iwatsuki K., Kaneko F. Novel point mutations in the steroid sulfatase gene in patients with X- linked ichthyosis: transfection analysis using the mutated genes. J. Invest. Dermatol. 114:1195-1199 (2000)

2. Matsumoto J., Ariyoshi N., Ishii I., Kitada M. Functional characterization of seven single-nucleotide polymorphisms of the steroid sulfatase gene found in a Japanese population. J. Hum. Genet. 58:267-272 (2013)

Subcellular Location

Cytoplasmic vesicle. Endoplasmic reticulum.

UNIPROT

SwissProt: P08842 Human

Synonyms

ARSC antibody

ARSC1 antibody

Arylsulfatase C antibody

Arylsulfatase C isozyme S antibody

ASC antibody

EC 3.1.6.2 antibody

ES antibody

Estrone sulfatase antibody

SSDD antibody

Steroid sulfatase (microsomal) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Steroid sulfatase on different lysates with Rabbit anti-Steroid sulfatase antibody (<a href="/products/HA722441" style="font-weight: bold;text-decoration: underline;">HA722441</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: LNCaP cell lysate (negative)<br />Lane 3: HepG2 cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: Huh7 cell lysate<br />Lane 6: T-47D cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 65 kDa<br />Observed band size: 65 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722441" style="font-weight: bold;text-decoration: underline;">HA722441</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of Steroid sulfatase on different lysates with Rabbit anti-Steroid sulfatase antibody (HA722441) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: LNCaP cell lysate (negative)
    Lane 3: HepG2 cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: Huh7 cell lysate
    Lane 6: T-47D cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 65 kDa
    Observed band size: 65 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722441) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Steroid sulfatase antibody (<a href="/products/HA722441" style="font-weight: bold;text-decoration: underline;">HA722441</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722441" style="font-weight: bold;text-decoration: underline;">HA722441</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Steroid sulfatase antibody (HA722441) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722441) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Steroid sulfatase antibody (<a href="/products/HA722441" style="font-weight: bold;text-decoration: underline;">HA722441</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722441" style="font-weight: bold;text-decoration: underline;">HA722441</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Steroid sulfatase antibody (HA722441) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722441) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"