CCR9 Recombinant Rabbit Monoclonal Antibody [JE00-35]
Catalog# HA722444
CCR9 Recombinant Rabbit Monoclonal Antibody [JE00-35]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CCR9 Recombinant Rabbit Monoclonal Antibody [JE00-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CCR9 aa 1-100 / 369.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 42 kDa
Positive Control
A549 cell lysate, Jurkat cell lysate, HepG2 cell lysate, mouse thymus tissue lysate, rat lung tissue lysate, rat thymus tissue lysate.
Conjugation
unconjugated
Clone Number
JE00-35
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
The protein encoded by this gene is a G protein-coupled receptor with seven transmembrane domains that belongs to the beta chemokine receptor family. Chemokines and their receptors are key regulators of thymocyte migration and maturation in normal and inflammation conditions. This gene is differentially expressed in T lymphocytes of the small intestine and colon, and its interaction with chemokine 25 contributes to intestinal intra-epithelial lymphocyte homing to the small intestine. This suggests a role for this gene in directing immune responses to different segments of the gastrointestinal tract. This gene and its exclusive ligand, chemokine 25, are overexpressed in a variety of malignant tumors and are closely associated with tumor proliferation, apoptosis, invasion, migration and drug resistance. This gene maps to the chemokine receptor gene cluster. Multiple transcript variants encoding different isoforms have been found for this gene.
Background References
1. Wu X et al. The Roles of CCR9/CCL25 in Inflammation and Inflammation-Associated Diseases. Front Cell Dev Biol. 2021 Aug
2. Jamal M et al. CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis. Front Pharmacol. 2023 Sep
Subcellular Location
Cell membrane.
Synonyms
C C chemokine receptor D6 antibody
C C chemokine receptor type 9 antibody
C C CKR 9 antibody
C-C chemokine receptor type 9 antibody
C-C CKR-9 antibody
CC Chemokine receptor CCR10 antibody
CC CKR 9 antibody
CC-CKR-9 antibody
CCBP2 antibody
CCCKR9 antibody
ExpandImages
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Western blot analysis of CCR9 on different lysates with Rabbit anti-CCR9 antibody (HA722444) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: Mouse thymus tissue lysate (40 µg/Lane)
Lane 5: Rat lung tissue lysate (40 µg/Lane)
Lane 6: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute 50 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722444) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"