INPP4B Recombinant Rabbit Monoclonal Antibody [JE07-42]
Catalog# HA722445
INPP4B Recombinant Rabbit Monoclonal Antibody [JE07-42]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
INPP4B Recombinant Rabbit Monoclonal Antibody [JE07-42]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human INPP4B aa 1-100.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 105 kDa
Positive Control
HeLa cell lysate, mouse heart tissue lysate, mouse skeletal muscle tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysate, rat heart tissue.
Conjugation
unconjugated
Clone Number
JE07-42
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
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1:200
Target
Function
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate. Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles. The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. INPP4B encodes the inositol polyphosphate 4-phosphatase type II, one of the enzymes involved in phosphatidylinositol signaling pathways. This enzyme removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate. There is limited data to suggest that the human type II enzyme is subject to alternative splicing, as has been established for the type I enzyme.
Background References
1. Lopez S.M., Hodgson M.C., Packianathan C., Bingol-Ozakpinar O., Uras F., Rosen B.P., Agoulnik I.U. Determinants of the tumor suppressor INPP4B protein and lipid phosphatase activities. Biochem. Biophys. Res. Commun. 440:277-282 (2013)
2. Maekawa M., Terasaka S., Mochizuki Y., Kawai K., Ikeda Y., Araki N., Skolnik E.Y., Taguchi T., Arai H. Sequential breakdown of 3-phosphorylated phosphoinositides is essential for the completion of macropinocytosis. Proc. Natl. Acad. Sci. U.S.A. 111:E978-E987 (2014)
Subcellular Location
Cytoplasm.
Synonyms
Inositol polyphosphate 4 phosphatase, type II antibody
4-bisphosphate 4-phosphatase antibody
Inositol polyphosphate 4 phosphatase type II 105kDa antibody
Inositol polyphosphate 4-phosphatase type II antibody
INP4B_HUMAN antibody
Inpp4b antibody
MGC132014 antibody
Type II inositol 3,4 bisphosphate 4 phosphatase antibody
Type II inositol-3 antibody
Images
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Western blot analysis of INPP4B on different lysates with Rabbit anti-INPP4B antibody (HA722445) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (40 µg/Lane)
Lane 3: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 4: Rat heart tissue lysate (40 µg/Lane)
Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)
Predicted band size: 107 kDa
Observed band size: 107 kDa
Exposure time: Lane 1: 30 seconds; Lane 2-5: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722445) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-INPP4B antibody (HA722445) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"