HIPK2 Recombinant Rabbit Monoclonal Antibody [JE37-78]
Catalog# HA722451
HIPK2 Recombinant Rabbit Monoclonal Antibody [JE37-78]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
HIPK2 Recombinant Rabbit Monoclonal Antibody [JE37-78]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human HIPK2 aa 131-230 / 1,198.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 131 kDa
Positive Control
Raji cell lysate, HepG2 cell lysate, Raji.
Conjugation
unconjugated
Clone Number
JE37-78
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:100
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FC
-
1:1,000
Target
Function
Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4 and DAZAP2. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. In response to DNA damage, phosphorylates DAZAP2 which localizes DAZAP2 to the nucleus, reduces interaction of DAZAP2 with HIPK2 and prevents DAZAP2-dependent ubiquitination of HIPK2 by E3 ubiquitin-protein ligase SIAH1 and subsequent proteasomal degradation
Background References
1. Liebl M.C., Moehlenbrink J., Becker H., Raddatz G., Abdeen S.K., Aqeilan R.I., Lyko F., Hofmann T.G. DAZAP2 acts as specifier of the p53 response to DNA damage. Nucleic Acids Res. 49:2759-2776 (2021)
Subcellular Location
Cytoplasm. Nucleus.
UNIPROT
Synonyms
hHIPk 2 antibody
hHIPk2 antibody
HIPK 2 antibody
Hipk2 antibody
HIPK2_HUMAN antibody
Homeodomain interacting protein kinase 2 antibody
Homeodomain-interacting protein kinase 2 antibody
Nbak1 antibody
Nuclear body-associated kinase 1 antibody
PRO0593 antibody
ExpandImages
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Western blot analysis of HIPK2 on different lysates with Rabbit anti-HIPK2 antibody (HA722451) at 1/1,000 dilution.
Lane 1: Raji cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Predicted band size: 131 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722451) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Raji cells labeling HIPK2 with Rabbit anti-HIPK2 antibody (HA722451) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIPK2 antibody (HA722451) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of Raji cells labeling HIPK2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722451, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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