Crk p38 Recombinant Rabbit Monoclonal Antibody [JE38-60]
Catalog# HA722453
Crk p38 Recombinant Rabbit Monoclonal Antibody [JE38-60]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
Crk p38 Recombinant Rabbit Monoclonal Antibody [JE38-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Crk p38 aa 1-100 / 304.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 34 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, C6 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE38-60
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Background References
1. Lopez M.L., Lo M., Kung J.E., Dudkiewicz M., Jang G.M., Von Dollen J., Johnson J.R., Krogan N.J., Pawlowski K., Jura N. PEAK3/C19orf35 pseudokinase, a new NFK3 kinase family member, inhibits CrkII through dimerization. Proc. Natl. Acad. Sci. U.S.A. 116:15495-15504 (2019)
2. Sanders M.A., Ampasala D., Basson M.D. DOCK5 and DOCK1 regulate Caco-2 intestinal epithelial cell spreading and migration on collagen IV. J. Biol. Chem. 284:27-35 (2009)
Subcellular Location
Cell membrane. Cytoplasm.
Synonyms
Adapter molecule crk antibody
Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
CRK antibody
CRK isoform 2 antibody
CRK isoform II antibody
CRK_HUMAN antibody
CRKII antibody
FLJ38130 antibody
OTTHUMP00000115366 antibody
OTTHUMP00000198330 antibody
ExpandImages
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Western blot analysis of Crk p38 on different lysates with Rabbit anti-Crk p38 antibody (HA722453) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: C6 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722453) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"