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ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]

Usd: 385

Specification

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Catalog# HA722455

ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]

Application

  • WB

  • IP

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ABO Recombinant Rabbit Monoclonal Antibody [JE39-25]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ABO aa 1-50 / 354.

Species Reactivity

Human, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 41 kDa

Positive Control

HT-29 cell lysate, K-562 cell lysate, 293T cell lysate, rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

JE39-25

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.

Background References

1. Letts J.A., Rose N.L., Fang Y.R., Barry C.H., Borisova S.N., Seto N.O.L., Palcic M.M., Evans S.V. Differential recognition of the type I and II H antigen acceptors by the human ABO(H) blood group A and B glycosyltransferases. J. Biol. Chem. 281:3625-3632 (2006)

2. Posch-Pertl L., List W., Michelitsch M., Pinter-Hausberger S., Posch F., Innauer F., Renner W., Weger M. Role of the ABO Blood Groups as a Risk Factor for Retinal Vein Occlusion. Ophthalmic Res 66:164-169 (2023)

Subcellular Location

Golgi apparatus, Golgi stack membrane. Secreted.

UNIPROT

SwissProt: P16442 Human

Synonyms

A transferase antibody

Abo antibody

B transferase antibody

BGAT_HUMAN antibody

Blood Group H2 antigen antibody

CD173 antibody

Fucosylglycoprotein 3-alpha-galactosyltransferase antibody

Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase antibody

Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form antibody

Glycoprotein-fucosylgalactoside alpha-galactosyltransferase antibody

Expand

Images

  • Western blot analysis of ABO on different lysates with Rabbit anti-ABO antibody (<a href="/products/HA722455" style="font-weight: bold;text-decoration: underline;">HA722455</a>) at 1/1,000 dilution.<br /><br />Lane 1: HT-29 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: 293T cell lysate (20 µg/Lane)<br />Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722455" style="font-weight: bold;text-decoration: underline;">HA722455</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ABO on different lysates with Rabbit anti-ABO antibody (HA722455) at 1/1,000 dilution.

    Lane 1: HT-29 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: 293T cell lysate (20 µg/Lane)
    Lane 4: Rat skeletal muscle tissue lysate (40 µg/Lane)

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722455) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ABO was immunoprecipitated from 0.2 mg 293T cell lysate with <a href="/products/HA722455" style="font-weight: bold;text-decoration: underline;">HA722455</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722455" style="font-weight: bold;text-decoration: underline;">HA722455</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: 293T cell lysate (input)<br />Lane 2: <a href="/products/HA722455" style="font-weight: bold;text-decoration: underline;">HA722455</a> IP in 293T cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722455" style="font-weight: bold;text-decoration: underline;">HA722455</a> in 293T cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 1 minute 2 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    ABO was immunoprecipitated from 0.2 mg 293T cell lysate with HA722455 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722455 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: 293T cell lysate (input)
    Lane 2: HA722455 IP in 293T cell lysate
    Lane 3: Rabbit IgG instead of HA722455 in 293T cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 1 minute 2 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"