SCIN Recombinant Rabbit Monoclonal Antibody [JE62-89]
Catalog# HA722465
SCIN Recombinant Rabbit Monoclonal Antibody [JE62-89]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SCIN Recombinant Rabbit Monoclonal Antibody [JE62-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SCIN aa 616-715 / 715.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 80 kDa
Positive Control
HEK-293 cell lysate, NIH/3T3 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate.
Conjugation
unconjugated
Clone Number
JE62-89
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Ca2+-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2) (By similarity). In vitro, also has barbed end capping and nucleating activities in the presence of Ca2+. Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles. Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts. Regulates chondrocyte proliferation and differentiation. Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways.
Background References
1. Zunino R., Li Q., Rose S.D., Romero-Benitez M.M., Lejen T., Brandan N.C., Trifaro J.M. Expression of scinderin in megakaryoblastic leukemia cells induces differentiation, maturation, and apoptosis with release of plateletlike particles and inhibits proliferation and tumorigenesis. Blood 98:2210-2219 (2001)
2. Marcu M.G., Zhang L., Nau-Staudt K., Trifaro J.M. Recombinant scinderin, an F-actin severing protein, increases calcium- induced release of serotonin from permeabilized platelets, an effect blocked by two scinderin-derived actin-binding peptides and phosphatidylinositol 4,5-bisphosphate. Blood 87:20-24 (1996)
Subcellular Location
Cytoplasm, cytoskeleton.
Synonyms
Adseverin antibody
ADSV_HUMAN antibody
KIAA1905 antibody
Scin antibody
Scinderin antibody
Images
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Western blot analysis of SCIN on different lysates with Rabbit anti-SCIN antibody (HA722465) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
Lane 4: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722465) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"