SAGE1 Recombinant Rabbit Monoclonal Antibody [PSH05-89]
Catalog# HA722487
SAGE1 Recombinant Rabbit Monoclonal Antibody [PSH05-89]
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WB
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IHC-P
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IF-Cell
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IP
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Human
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HA751036
不含抗保成分
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unconjugated
Overview
Product Name
SAGE1 Recombinant Rabbit Monoclonal Antibody [PSH05-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human SAGE1 aa 75-733.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell, IP
Molecular Weight
Predicted band size: 99 kDa
Positive Control
K-562 cell lysates, K-562, human testis tissue.
Conjugation
unconjugated
Clone Number
PSH05-89
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:500
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IP
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1-2μg/sample
Target
Function
This gene belongs to a class of genes that are activated in tumors. These genes are expressed in tumors of different histologic types but not in normal tissues, except for spermatogenic cells and, for some, placenta. The proteins encoded by these genes appear to be strictly tumor specific, and hence may be excellent sources of antigens for cancer immunotherapy. This gene is expressed in sarcomas.
Background References
1. Miyahara Y., Naota H., Wang L., Hiasa A., Goto M., Watanabe M., Kitano S., Okumura S., Takemitsu T., Shiku H. Determination of cellularly processed HLA-A2402-restricted novel CTL epitopes derived from two cancer germ line genes, MAGE-A4 and SAGE. Clin Cancer Res 11:5581-5589 (2005)
2. Maheswaran E., Pedersen C.B., Ditzel H.J., Gjerstorff M.F. Lack of ADAM2, CALR3 and SAGE1 Cancer/Testis Antigen Expression in Lung and Breast Cancer. PLoS One 10:e0134967-e0134967 (2015)
Subcellular Location
Nuclear body. Nucleoplasm.
UNIPROT
Synonyms
Cancer/testis antigen 14 antibody
CT14 antibody
RP11-97N5.1 antibody
SAGE antibody
SAGE1 antibody
SAGE1_HUMAN antibody
Sarcoma antigen 1 antibody
Images
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Western blot analysis of SAGE1 on K-562 cell lysates with Rabbit anti-SAGE1 antibody (HA722487) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 99 kDa
Observed band size: 120 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722487) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of K-562 cells labeling SAGE1 with Rabbit anti-SAGE1 antibody (HA722487) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SAGE1 antibody (HA722487) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SAGE1 antibody (HA722487) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722487) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
SAGE1 was immunoprecipitated from 0.2 mg K-562 cell lysate with HA722487 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722487 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate (input)
Lane 2: HA722487 IP in K-562 cell lysate
Lane 3: Rabbit IgG instead of HA722487 in K-562 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"