SESN2 / Sestrin-2 Recombinant Rabbit Monoclonal Antibody [PSH05-78]
Catalog# HA722489
SESN2 / Sestrin-2 Recombinant Rabbit Monoclonal Antibody [PSH05-78]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SESN2 / Sestrin-2 Recombinant Rabbit Monoclonal Antibody [PSH05-78]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SESN2 aa 231-280.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 54 kDa
Positive Control
K-562 cell lysate, HEK-293 cell lysate, 293T cell lysate, HCT 116 cell lysate, LoVo cell lysate, HepG2 cell lysate, Jurkat cell lysate, U-87 MG cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
PSH05-78
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Sestrin-2 also known as Hi95 is a protein that in humans is encoded by the SESN2 gene. This gene encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions. The Sestrins constitute a family of evolutionarily-conserved stress-inducible proteins that suppress oxidative stress and regulate adenosine monophosphate-dependent protein kinase (AMPK)-mammalian target of rapamycin (mTOR) signaling. By virtue of these activities, the Sestrins serve as important regulators of metabolic homeostasis. Accordingly, inactivation of Sestrin genes in invertebrates resulted in diverse metabolic pathologies, including oxidative damage, fat accumulation, mitochondrial dysfunction and muscle degeneration that resemble accelerated tissue aging.
Background References
1. Xu L et al. SESN2 Could Be a Potential Marker for Diagnosis and Prognosis in Glioma. Genes (Basel). 2023 Mar
2. Wang B J et al. Regulatory mechanisms of Sesn2 and its role in multi-organ diseases. Pharmacol Res. 2021 Feb
Subcellular Location
Cytoplasm.
Synonyms
DKFZp761M0212 antibody
DKFZp761M02121 antibody
Hi95 antibody
Hypoxia induced gene 95 antibody
MGC11758 antibody
OTTHUMP00000003641 antibody
OTTMUSP00000010184 antibody
RP23 63D8.3 antibody
SES2 antibody
SESN 2 antibody
ExpandImages
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Western blot analysis of SESN2 / Sestrin-2 on different lysates with Rabbit anti-SESN2 / Sestrin-2 antibody (HA722489) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: 293T cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: LoVo cell lysate
Lane 6: HepG2 cell lysate
Lane 7: Jurkat cell lysate
Lane 8: U-87 MG cell lysate
Lane 9: NIH/3T3 cell lysate
Lane 10: RAW264.7 cell lysate
Lane 11: C6 cell lysate
Lane 12: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: Lane 1-8: 1 minute 18 seconds; Lane 9-12: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722489) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"