Phospho-FOXO1A (T24)+ FOXO3A (T32)+ FOXO4 (T28)+ FOXO6 (T26) Recombinant Rabbit Monoclonal Antibody [PSH06-00] (HA722499)
Catalog# HA722499
Rabbit Monoclonal to Phospho-FOXO1A (T24)+ FOXO3A (T32)+ FOXO4 (T28)+ FOXO6 (T26)
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Phospho-FOXO1A (T24)+ FOXO3A (T32)+ FOXO4 (T28)+ FOXO6 (T26) on different lysates with Rabbit anti-Phospho-FOXO1A (T24)+ FOXO3A (T32)+ FOXO4 (T28)+ FOXO6 (T26) antibody (HA722499) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat treated with 20μM LY294002 for 2 hours cell lysate
Lane 3: Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate
Lane 4: NIH/3T3 starved for 24 hours cell lysate
Lane 5: NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: 55-150 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722499) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: Jurkat treated with 20μM LY294002 for 2 hours cell lysate
Lane 3: Jurkat treated with 100nM Calyculin A for 40 minutes cell lysate
Lane 4: NIH/3T3 starved for 24 hours cell lysate
Lane 5: NIH/3T3 starved for 24 hours then add 100nM Calyculin A for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Observed band size: 55-150 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722499) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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