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MX1 Recombinant Rabbit Monoclonal Antibody [PSH06-03]

Usd: 385

Specification

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Catalog# HA722502

MX1 Recombinant Rabbit Monoclonal Antibody [PSH06-03]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

MX1 Recombinant Rabbit Monoclonal Antibody [PSH06-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MX1 aa 351-662.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 76 kDa

Positive Control

HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate, Daudi treated with 10ng/mL IFN-α1 for 16 hours, human spleen tissue.

Conjugation

unconjugated

Clone Number

PSH06-03

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:200

Target

Function

Interferon-induced GTP-binding protein Mx1 is a protein that in humans is encoded by the MX1 gene. In mice, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. Furthermore, the human orthologue MxA is a major determinant for influenza viruses of animal origin. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin superfamily and the family of large GTPases.

Background References

1. Chen Y et al. Rare variant MX1 alleles increase human susceptibility to zoonotic H7N9 influenza virus. Science. 2021 Aug

2. McKellar J et al. MX1 proteins and influenza A virus under the microscope. Virologie (Montrouge). 2023 Feb

Subcellular Location

Cytoplasm, Endoplasmic reticulum membrane, Cytoplasm, perinuclear region; Cytoplasm, Nucleus.

UNIPROT

SwissProt: P20591 Human

Synonyms

Human interferon regulated resistance GTP binding protein MXA antibody

IFI 78 antibody

IFI 78K antibody

IFI-78K antibody

IFI78 antibody

IFI78K antibody

Interferon induced GTP binding protein Mx1 antibody

Interferon induced protein p78 antibody

Interferon inducible protein p78 antibody

Interferon regulated resistance GTP binding protein antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of MX1 on different lysates with Rabbit anti-MX1 antibody (<a href="/products/HA722502" style="font-weight: bold;text-decoration: underline;">HA722502</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate<br />Lane 3: Daudi cell lysate<br />Lane 4: Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 76 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 17 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722502" style="font-weight: bold;text-decoration: underline;">HA722502</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of MX1 on different lysates with Rabbit anti-MX1 antibody (HA722502) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate
    Lane 3: Daudi cell lysate
    Lane 4: Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 76 kDa
    Observed band size: 70 kDa

    Exposure time: 17 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722502) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of Daudi cells treated with 10ng/mL IFN-α1 for 16 hours labeling MX1 with Rabbit anti-MX1 antibody (<a href="/products/HA722502" style="font-weight: bold;text-decoration: underline;">HA722502</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MX1 antibody (<a href="/products/HA722502" style="font-weight: bold;text-decoration: underline;">HA722502</a>) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of Daudi cells treated with 10ng/mL IFN-α1 for 16 hours labeling MX1 with Rabbit anti-MX1 antibody (HA722502) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MX1 antibody (HA722502) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MX1 antibody (<a href="/products/HA722502" style="font-weight: bold;text-decoration: underline;">HA722502</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722502" style="font-weight: bold;text-decoration: underline;">HA722502</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MX1 antibody (HA722502) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722502) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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