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IFNGR1 Recombinant Rabbit Monoclonal Antibody [PSH06-11]

Usd: 385

Specification

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Catalog# HA722508

IFNGR1 Recombinant Rabbit Monoclonal Antibody [PSH06-11]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

IFNGR1 Recombinant Rabbit Monoclonal Antibody [PSH06-11]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human IFNGR1 aa 18-245 (Extracellular).

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 54 kDa

Positive Control

NCI-H226 cell lysate, LoVo cell lysate, HepG2 cell lysate, THP-1 cell lysate, MCF7 cell lysate, K-562 cell lysate, SW620 cell lysate, HL-60 cell lysate, LoVo.

Conjugation

unconjugated

Clone Number

PSH06-11

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription. STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. A form of Mendelian susceptibility to mycobacterial disease, a rare condition caused by impairment of interferon-gamma mediated immunity. It is characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine, environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. Clinical outcome severity depends on the degree of impairment of interferon-gamma mediated immunity. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas.

Background References

1. Du W et al. Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation. Cancer Discov. 2021 Jul

2. Singh S et al. Loss of ELF5-FBXW7 stabilizes IFNGR1 to promote the growth and metastasis of triple-negative breast cancer through interferon-γ signalling. Nat Cell Biol. 2020 May

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P15260 Human

Synonyms

Antiviral Protein Type II antibody

Antiviral protein, type 2 antibody

AVP type II antibody

AVP, type 2 antibody

CD 119 antibody

CD119 antibody

CD119 antigen antibody

CDw119 antibody

FLJ45734 antibody

IFN gamma R alpha antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (<a href="/products/HA722508" style="font-weight: bold;text-decoration: underline;">HA722508</a>) at 1/1,000 dilution.<br /><br />Lane 1: NCI-H226 cell lysate<br />Lane 2: LoVo cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: THP-1 cell lysate<br />Lane 5: MCF7 cell lysate (low expression)<br />Lane 6: K-562 cell lysate<br />Lane 7: SW620 cell lysate<br />Lane 8: HL-60 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 40-100 kDa<br /><br />Exposure time: Lane 1: 20 seconds; Lane 2-8: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722508" style="font-weight: bold;text-decoration: underline;">HA722508</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA722508) at 1/1,000 dilution.

    Lane 1: NCI-H226 cell lysate
    Lane 2: LoVo cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: THP-1 cell lysate
    Lane 5: MCF7 cell lysate (low expression)
    Lane 6: K-562 cell lysate
    Lane 7: SW620 cell lysate
    Lane 8: HL-60 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 40-100 kDa

    Exposure time: Lane 1: 20 seconds; Lane 2-8: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722508) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody (<a href="/products/HA722508" style="font-weight: bold;text-decoration: underline;">HA722508</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody (<a href="/products/HA722508" style="font-weight: bold;text-decoration: underline;">HA722508</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody (HA722508) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody (HA722508) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (<a href="/products/HA722508" style="font-weight: bold;text-decoration: underline;">HA722508</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-IFNGR1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 45/120 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722508" style="font-weight: bold;text-decoration: underline;">HA722508</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA722508) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-IFNGR1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 45/120 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722508) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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