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HRP Conjugated Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39]

Usd: 409

Specification

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Catalog# HA722518

HRP Conjugated Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39]

Application

  • WB

  • IHC-P

  • ELISA

Reactivity

  • Species independent

Conjugation

  • HRP

Overview

Product Name

HRP Conjugated Digoxigenin Recombinant Rabbit Monoclonal Antibody [PSH04-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

DIG-OVA

Species Reactivity

Species independent

Validated Applications

WB, IHC-P, ELISA

Conjugation

HRP

Clone Number

PSH04-39

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:200-1:5,000

  • ELISA

  • 1:5,000-1:20,000

Target

Function

Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. digoxin, lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2,4-Dinitrophenol, biotin, and fluorescein. Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin interaction).

Background References

1. Barratt KS et al. Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments. Curr Protoc Mouse Biol. 2020 Jun

2. Tanji M et al. Digoxigenin-labeled RNA probes for untranslated regions enable the isoform-specific gene expression analysis of myosin heavy chains in whole-mount in situ hybridization. Dev Growth Differ. 2023 Jan

Synonyms

1672-46-4 antibody

BRN 0096479 antibody

DIG antibody

EINECS 216-806-2 antibody

HSDB 7108 antibody

Lanadigenin antibody

Lanadigigenin antibody

ST056392 antibody

Digoxigenin

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/2,000 dilution.<br /><br />Lane 1: DIG-BSA (2 ng/Lane)<br />Lane 2: BSA (negative) (2 ng/Lane)<br />Lane 3: 293T cell lysate (negative) (20 µg/Lane)<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight.

    ☑ Relative expression (RE)

    Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (HA722518) at 1/2,000 dilution.

    Lane 1: DIG-BSA (2 ng/Lane)
    Lane 2: BSA (negative) (2 ng/Lane)
    Lane 3: 293T cell lysate (negative) (20 µg/Lane)

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (HA722518) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722518) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (HA722518) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722518) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (HA722518) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722518) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722518" style="font-weight: bold;text-decoration: underline;">HA722518</a>) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue (negative) with Rabbit anti-Digoxigenin antibody (HA722518) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722518) at 1/1,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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