PKM1/2 Recombinant Rabbit Monoclonal Antibody [JE07-34]
Catalog# HA722524
PKM1/2 Recombinant Rabbit Monoclonal Antibody [JE07-34]
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WB
-
IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PKM1/2 Recombinant Rabbit Monoclonal Antibody [JE07-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PKM aa 50-100.
Product Specificity
PKM1/2 Recombinant Rabbit Monoclonal Antibody [JE07-34] detects endogenous levels of total PKM2 protein and PKM1 protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 58 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate.
Conjugation
unconjugated
Clone Number
JE07-34
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
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IP
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1-2μg/sample
Target
Function
Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene. PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells.
Background References
1. Ma WK et al. ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth. Cancer Res. 2022 Mar
2. Kejiou NS et al. Pyruvate Kinase M (PKM) binds ribosomes in a poly-ADP ribosylation dependent manner to induce translational stalling. Nucleic Acids Res. 2023 Jul
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
CTHBP antibody
Cytosolic thyroid hormone-binding protein antibody
KPYM_HUMAN antibody
OIP-3 antibody
Opa-interacting protein 3 antibody
p58 antibody
pkm antibody
PKM1 antibody
PKM2 antibody
Pyruvate kinase 2/3 antibody
ExpandImages
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Western blot analysis of PKM1/2 on different lysates with Rabbit anti-PKM1/2 antibody (HA722524) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (30 µg/Lane)
Lane 7: Rat skeletal muscle tissue lysate (30 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722524) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
PKM1/2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722524 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722524 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA722524 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA722524 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"