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CDCA3 Recombinant Rabbit Monoclonal Antibody [JE08-65]

Usd: 385

Specification

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Catalog# HA722525

CDCA3 Recombinant Rabbit Monoclonal Antibody [JE08-65]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CDCA3 Recombinant Rabbit Monoclonal Antibody [JE08-65]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CDCA3 aa 1-50 / 268.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 29 kDa

Positive Control

Caco-2 cell lysate, A431 cell lysate, HDLM-2 cell lysate, A549 cell lysate, SiHa cell lysate.

Conjugation

unconjugated

Clone Number

JE08-65

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity). Predicted to be involved in cell division and protein ubiquitination. Located in adherens junction.

Background References

1. Gong S et al. CDCA3 exhibits a role in promoting the progression of ovarian cancer. Tissue Cell. 2022 Dec

2. Bai Y et al. CDCA3 Predicts Poor Prognosis and Affects CD8(+) T Cell Infiltration in Renal Cell Carcinoma. J Oncol. 2022 Sep

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: Q99618 Human

Synonyms

C8 antibody

CDCA 3 antibody

Cell division cycle associated 3 antibody

Cell division cycle associated protein 3 antibody

Gene rich cluster C8 antibody

Gene rich cluster protein C8 antibody

GRCC 8 antibody

GRCC8 antibody

MGC2577 antibody

TOME 1 antibody

Expand

Images

  • Western blot analysis of CDCA3 on different lysates with Rabbit anti-CDCA3 antibody (<a href="/products/HA722525" style="font-weight: bold;text-decoration: underline;">HA722525</a>) at 1/1,000 dilution.<br /><br />Lane 1: Caco-2 cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: HDLM-2 cell lysate<br />Lane 4: A549 cell lysate<br />Lane 5: SiHa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 29 kDa<br />Observed band size: 29 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722525" style="font-weight: bold;text-decoration: underline;">HA722525</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CDCA3 on different lysates with Rabbit anti-CDCA3 antibody (HA722525) at 1/1,000 dilution.

    Lane 1: Caco-2 cell lysate
    Lane 2: A431 cell lysate
    Lane 3: HDLM-2 cell lysate
    Lane 4: A549 cell lysate
    Lane 5: SiHa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722525) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"