ITCH / AIP4 Recombinant Rabbit Monoclonal Antibody [JE31-09]
Catalog# HA722530
ITCH / AIP4 Recombinant Rabbit Monoclonal Antibody [JE31-09]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ITCH / AIP4 Recombinant Rabbit Monoclonal Antibody [JE31-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human ITCH / AIP4 aa 1-50 / 903.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 103 kDa
Positive Control
SiHa cell lysate, HeLa cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE31-09
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
Target
Function
ITCH is a HECT domain–containing E3 ubiquitin ligase that is ablated in non-agouti-lethal 18H (aka Itchy) mice. Itchy mice develop a severe immunological phenotype after birth that includes hyperplasia of lymphoid and hematopoietic cells, and stomach and lung inflammation. In humans ITCH deficiency causes altered physical growth, craniofacial morphology defects, defective muscle development, and aberrant immune system function. The ITCH gene is located on chromosome 20 in humans. ITCH contains a C2 domain, proline-rich region, WW domains, HECT domain, and multiple amino acids that are phosphorylated and ubiquitinated.
Background References
1. Cevikbas F et al. Physiology and Pathophysiology of Itch. Physiol Rev. 2020 Jul
2. Roh YS et al. Itch: Epidemiology, clinical presentation, and diagnostic workup. J Am Acad Dermatol. 2022 Jan
Subcellular Location
Cell membrane, Cytoplasm, Nucleus, Early endosome membrane, Endosome membrane.
Synonyms
ADMFD antibody
AIF4 antibody
AIP4 antibody
Atrophin 1 interacting protein 4 antibody
Atrophin-1-interacting protein 4 antibody
dJ468O1.1 antibody
dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) antibody
dJ468O1.1 atrophin 1 interacting protein 4 AIP4 antibody
E3 ubiquitin protein ligase Itchy homolog antibody
E3 ubiquitin-protein ligase Itchy homolog antibody
ExpandImages
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Western blot analysis of ITCH / AIP4 on different lysates with Rabbit anti-ITCH / AIP4 antibody (HA722530) at 1/1,000 dilution.
Lane 1: SiHa cell lysate
Lane 2: HeLa cell lysate
Lane 3: Ramos cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 103 kDa
Observed band size: 103 kDa
Exposure time: 45 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722530) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ITCH / AIP4 on different lysates with Rabbit anti-ITCH / AIP4 antibody (HA722530) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-ITCH / AIP4 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 90 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722530) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"