CD82 Recombinant Rabbit Monoclonal Antibody [JE32-17]
Catalog# HA722533
CD82 Recombinant Rabbit Monoclonal Antibody [JE32-17]
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WB
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Human
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unconjugated
Overview
Product Name
CD82 Recombinant Rabbit Monoclonal Antibody [JE32-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CD82 aa 251-267 / 267.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 30 kDa
Positive Control
Jurkat cell lysate, U-87 MG cell lysate, SW1990 cell lysate, Raji cell lysate.
Conjugation
unconjugated
Clone Number
JE32-17
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
CD82 (Cluster of Differentiation 82), or KAI1, is a human protein encoded by the CD82 gene. This metastasis suppressor gene product is a membrane glycoprotein that is a member of the tetraspanin/transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Background References
1. Wang S et al. KAI1/CD82 gene and autotaxin-lysophosphatidic acid axis in gastrointestinal cancers. World J Gastrointest Oncol. 2022 Aug
2. Viera M et al. Targeting CD82/KAI1 for Precision Therapeutics in Surmounting Metastatic Potential in Breast Cancer. Cancers (Basel). 2021 Sep
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
4F9 antibody
Antigen detected by monoclonal and antibody IA4 antibody
C33 antibody
C33 antigen antibody
CD 82 antibody
CD82 antibody
CD82 antigen antibody
CD82 molecule antibody
CD82_HUMAN antibody
GR 15 antibody
ExpandImages
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Western blot analysis of CD82 on different lysates with Rabbit anti-CD82 antibody (HA722533) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: SW1990 cell lysate
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 30-50 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722533) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of CD82 on different lysates with Rabbit anti-CD82 antibody (HA722533) at 1/1,000 dilution.
Lane 1: Raji cell lysate
Lane 2: Raji cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 30-50 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722533) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"