Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]
Catalog# HA722537
Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]
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WB
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Human
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unconjugated
Overview
Product Name
Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Guanylyl Cyclase alpha 1 aa 1-200 / 690.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 77 kDa
Positive Control
HEK-293 cell lysate, SH-SY5Y cell lysate.
Conjugation
unconjugated
Clone Number
JE34-21
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs. Mutations in this gene have been associated to cases of myocardial infarction.
Background References
1. Korkmaz Y et al. Dental Pulp Inflammation Initiates the Occurrence of Mast Cells Expressing the alpha(1) and beta(1) Subunits of Soluble Guanylyl Cyclase. Int J Mol Sci. 2023 Jan
2. Guey S et al. Biallelic variants in NOS3 and GUCY1A3, the two major genes of the nitric oxide pathway, cause moyamoya cerebral angiopathy. Hum Genomics. 2023 Mar
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
GC S alpha 1 antibody
GC SA3 antibody
GCS alpha 1 antibody
GCS alpha 3 antibody
GCS-alpha-1 antibody
GCS-alpha-3 antibody
GCYA3_HUMAN antibody
Guanylate cyclase 1 soluble alpha 3 antibody
Guanylate cyclase soluble subunit alpha 1 antibody
Guanylate cyclase soluble subunit alpha 3 antibody
ExpandImages
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Western blot analysis of Guanylyl Cyclase alpha 1 on different lysates with Rabbit anti-Guanylyl Cyclase alpha 1 antibody (HA722537) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 75 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"