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Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]

Usd: 385

Specification

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Catalog# HA722537

Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Guanylyl Cyclase alpha 1 aa 1-200 / 690.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 77 kDa

Positive Control

HEK-293 cell lysate, SH-SY5Y cell lysate.

Conjugation

unconjugated

Clone Number

JE34-21

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs. Mutations in this gene have been associated to cases of myocardial infarction.

Background References

1. Korkmaz Y et al. Dental Pulp Inflammation Initiates the Occurrence of Mast Cells Expressing the alpha(1) and beta(1) Subunits of Soluble Guanylyl Cyclase. Int J Mol Sci. 2023 Jan

2. Guey S et al. Biallelic variants in NOS3 and GUCY1A3, the two major genes of the nitric oxide pathway, cause moyamoya cerebral angiopathy. Hum Genomics. 2023 Mar

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: Q02108 Human

Synonyms

GC S alpha 1 antibody

GC SA3 antibody

GCS alpha 1 antibody

GCS alpha 3 antibody

GCS-alpha-1 antibody

GCS-alpha-3 antibody

GCYA3_HUMAN antibody

Guanylate cyclase 1 soluble alpha 3 antibody

Guanylate cyclase soluble subunit alpha 1 antibody

Guanylate cyclase soluble subunit alpha 3 antibody

Expand

Images

  • Western blot analysis of Guanylyl Cyclase alpha 1 on different lysates with Rabbit anti-Guanylyl Cyclase alpha 1 antibody (<a href="/products/HA722537" style="font-weight: bold;text-decoration: underline;">HA722537</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: SH-SY5Y cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 77 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 59 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722537" style="font-weight: bold;text-decoration: underline;">HA722537</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Guanylyl Cyclase alpha 1 on different lysates with Rabbit anti-Guanylyl Cyclase alpha 1 antibody (HA722537) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: SH-SY5Y cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 77 kDa
    Observed band size: 75 kDa

    Exposure time: 59 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"