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NPR-C Recombinant Rabbit Monoclonal Antibody [JE34-50]

Usd: 385

Specification

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Catalog# HA722538

NPR-C Recombinant Rabbit Monoclonal Antibody [JE34-50]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

NPR-C Recombinant Rabbit Monoclonal Antibody [JE34-50]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human NPR-C aa 505-541 / 541.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 60 kDa

Positive Control

HeLa cell lysate, Human lung tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE34-50

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C), also known as NPR3, is an atrial natriuretic peptide receptor. In humans it is encoded by the NPR3 gene. The family of natriuretic peptides elicit a number of vascular, renal, and endocrine effects that are important in the maintenance of blood pressure and extracellular fluid volume. These effects are mediated by specific binding of the peptides to cell surface receptors in the vasculature, kidney, adrenal, and brain.

Background References

1. Meng L et al. NPRC deletion attenuates cardiac fibrosis in diabetic mice by activating PKA/PKG and inhibiting TGF-beta1/Smad pathways. Sci Adv. 2023 Aug

2. Cheng C et al. NPRC deletion mitigated atherosclerosis by inhibiting oxidative stress, inflammation and apoptosis in ApoE knockout mice. Signal Transduct Target Ther. 2023 Aug

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P17342 Human

SwissProt: P70180 Mouse

SwissProt: P41740 Rat

Synonyms

ANP C antibody

ANP-C antibody

ANPC antibody

ANPR-C antibody

ANPRC antibody

ANPRC_HUMAN antibody

Atrial natriuretic peptide C type receptor antibody

Atrial natriuretic peptide clearance receptor antibody

Atrial natriuretic peptide receptor 3 antibody

Atrial natriuretic peptide receptor type C antibody

Expand

Images

  • Western blot analysis of NPR-C on different lysates with Rabbit anti-NPR-C antibody (<a href="/products/HA722538" style="font-weight: bold;text-decoration: underline;">HA722538</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Human lung tissue lysate (40 µg/Lane)<br />Lane 3: Mouse kidney tissue lysate (40 µg/Lane)<br />Lane 4: Rat kidney tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 60 kDa<br />Observed band size: 60/70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722538" style="font-weight: bold;text-decoration: underline;">HA722538</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NPR-C on different lysates with Rabbit anti-NPR-C antibody (HA722538) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Human lung tissue lysate (40 µg/Lane)
    Lane 3: Mouse kidney tissue lysate (40 µg/Lane)
    Lane 4: Rat kidney tissue lysate (40 µg/Lane)

    Predicted band size: 60 kDa
    Observed band size: 60/70 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722538) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling NPR-C.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722538" style="font-weight: bold;text-decoration: underline;">HA722538</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling NPR-C.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722538, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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