VPS24 Recombinant Rabbit Monoclonal Antibody [JE34-58]
Catalog# HA722540
VPS24 Recombinant Rabbit Monoclonal Antibody [JE34-58]
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WB
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
VPS24 Recombinant Rabbit Monoclonal Antibody [JE34-58]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human VPS24 aa 50-222 / 222.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 29 kDa
Positive Control
HepG2 cell lysate, U-87 MG cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE34-58
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Charged multivesicular body protein 3 is a protein that in humans is encoded by the VPS24 gene. This gene encodes a protein that acts in the sorting of transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants encoding different isoforms.
Background References
1. Banjade S et al. Design principles of the ESCRT-III Vps24-Vps2 module. Elife. 2021 May
2. Florian JR et al. Genetic analysis of the Drosophila ESCRT-III complex protein, VPS24, reveals a novel function in lysosome homeostasis. PLoS One. 2021 May
Subcellular Location
Cytoplasm, cytosol, Membrane, Endosome, Late endosome membrane.
Synonyms
VPS 24 antibody
VPS24 antibody
CGI 149 antibody
CGI149 protein antibody
Charged multivesicular body protein 3 antibody
CHMP3 antibody
CHMP3_HUMAN antibody
Chromatin modifying protein 3 antibody
Chromatin-modifying protein 3 antibody
HGNC:29865 antibody
ExpandImages
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Western blot analysis of VPS24 on different lysates with Rabbit anti-VPS24 antibody (HA722540) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: Human brain tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722540) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"