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CD52 Recombinant Rabbit Monoclonal Antibody [JE37-24]

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Specification

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Catalog# HA722545

CD52 Recombinant Rabbit Monoclonal Antibody [JE37-24]

Application

  • WB

  • FC

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

CD52 Recombinant Rabbit Monoclonal Antibody [JE37-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within mouse CD52 aa 1-47 / 74.

Species Reactivity

Mouse

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 8 kDa

Positive Control

Mouse spleen tissue lysate, mouse thymus tissue lysate, mouse spleen cells.

Conjugation

unconjugated

Clone Number

JE37-24

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC
mouse
Tested Tested
Not Recommended
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

CD52 is present on the surface of mature lymphocytes, but not on the stem cells from which these lymphocytes were derived. It also is found on monocytes and dendritic cells. Further, it is found within the male genital tract and is present on the surface of mature sperm cells. CD52 is a peptide of 12 amino acids, anchored to glycosylphosphatidylinositol (GPI). Since it is highly negatively charged and present on sperm cells and lymphocytes, it has been conjectured that its function is anti-adhesion, allowing cells to freely move around. CD52 binds the ITIM (immunoreceptor tyrosine-based inhibitory motif)-bearing sialic acid-binding lectin SIGLEC10.

Background References

1. Kasarello K et al. Anti-CD52 Therapy for Multiple Sclerosis: An Update in the COVID Era. Immunotargets Ther. 2021

2. de Vos-Hillebrand L et al. CD52 mRNA expression predicts prognosis and response to immune checkpoint blockade in melanoma. Pigment Cell Melanoma Res. 2024 Mar

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q64389 Mouse

Synonyms

Cambridge pathology 1 antigen antibody

CAMPATH 1 antibody

CAMPATH 1 antigen antibody

CAMPATH-1 antigen antibody

CD 52 antibody

CD52 antibody

CD52 antigen antibody

CD52 molecule antibody

CD52_HUMAN antibody

CDw52 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD52 on different lysates with Rabbit anti-CD52 antibody (<a href="/products/HA722545" style="font-weight: bold;text-decoration: underline;">HA722545</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse thymus tissue lysate<br />Lane 3: Mouse liver tissue lysate (negative)<br />Lane 4: Mouse kidney tissue lysate (negative)<br />Lane 5: Mouse brain tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 8 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722545" style="font-weight: bold;text-decoration: underline;">HA722545</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD52 on different lysates with Rabbit anti-CD52 antibody (HA722545) at 1/1,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse thymus tissue lysate
    Lane 3: Mouse liver tissue lysate (negative)
    Lane 4: Mouse kidney tissue lysate (negative)
    Lane 5: Mouse brain tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 8 kDa
    Observed band size: 16 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722545) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of mouse spleen cells labelling CD52 (<a href="/products/HA722545" style="font-weight: bold;text-decoration: underline;">HA722545</a>).

    Flow cytometric analysis of mouse spleen cells labelling CD52 (HA722545).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"