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PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]

Usd: 385

Specification

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Catalog# HA722554

PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PITX3 aa 1-50 / 302.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 32 kDa

Positive Control

HEK-293 cell lysate, SH-SY5Y cell lysate, C6 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat spleen tissue lysate.

Conjugation

unconjugated

Clone Number

JE41-12

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Pituitary homeobox 3 is a protein that in humans is encoded by the PITX3 gene. This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development, and the specification and terminal differentiation of mesencephalic dopamine neurons in the substantia nigra compacta that are lost in Parkinson's disease. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis (ASMD) and congenital cataracts.

Background References

1. Wang X et al. The Crucial Roles of Pitx3 in Midbrain Dopaminergic Neuron Development and Parkinson\'s Disease-Associated Neurodegeneration. Int J Mol Sci. 2023 May

2. Chen X et al. Pitx3 deficiency promotes age-dependent alterations in striatal medium spiny neurons. Front Aging Neurosci. 2022 Sep

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O75364 Human

SwissProt: O35160 Mouse

SwissProt: P81062 Rat

Synonyms

Homeobox protein PITX 3 antibody

Homeobox protein PITX3 antibody

MGC12766 antibody

Paired like homeodomain transcription factor 3 antibody

Paired-like homeodomain transcription factor 3 antibody

Pituitary homeobox 3 antibody

PITX 3 antibody

Pitx3 antibody

PITX3_HUMAN antibody

PTX 3 antibody

Expand

Images

  • Western blot analysis of PITX3 on different lysates with Rabbit anti-PITX3 antibody (<a href="/products/HA722554" style="font-weight: bold;text-decoration: underline;">HA722554</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate (20 µg/Lane)<br />Lane 2: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 3: C6 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (20 µg/Lane)<br />Lane 5: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 6: Mouse lung tissue lysate (40 µg/Lane)<br />Lane 7: Mouse spleen tissue lysate (40 µg/Lane)<br />Lane 8: Rat brain tissue lysate (40 µg/Lane)<br />Lane 9: Rat lung tissue lysate (40 µg/Lane)<br />Lane 10: Rat spleen tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 32 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722554" style="font-weight: bold;text-decoration: underline;">HA722554</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PITX3 on different lysates with Rabbit anti-PITX3 antibody (HA722554) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate (20 µg/Lane)
    Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 3: C6 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (20 µg/Lane)
    Lane 5: Mouse brain tissue lysate (40 µg/Lane)
    Lane 6: Mouse lung tissue lysate (40 µg/Lane)
    Lane 7: Mouse spleen tissue lysate (40 µg/Lane)
    Lane 8: Rat brain tissue lysate (40 µg/Lane)
    Lane 9: Rat lung tissue lysate (40 µg/Lane)
    Lane 10: Rat spleen tissue lysate (40 µg/Lane)

    Predicted band size: 32 kDa
    Observed band size: 35 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722554) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"