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SAHH Recombinant Rabbit Monoclonal Antibody [JE41-31]

Usd: 385

Specification

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Catalog# HA722555

SAHH Recombinant Rabbit Monoclonal Antibody [JE41-31]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SAHH Recombinant Rabbit Monoclonal Antibody [JE41-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human SAHH aa 351-400 / 432.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 48 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Jurkat cell lysate, Mouse liver tissue lysate, Mouse pancreas tissue lysate, Rat kidney tissue lysate, mouse lung tissue, rat lung tissue.

Conjugation

unconjugated

Clone Number

JE41-31

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

AdoHcyase is encoded by the AHCY gene in humans, which is believed to have a prognostic role in neuroblastoma. AdoHcyase is significantly associated with adenosine deaminase deficiency, which classically manifests in severe combine immunodeficiency (SCID). Accumulated adenosine derivatives, dATPs, irreversibly bind to and inhibit AdoHcyase, promoting the buildup of S-adenosyl-L-homocystine (due to equilibrium constant favors S-adenosyl-L-homocystine), a potent inhibitor of methyl transfer reactions.

Background References

1. Fan SH et al. Reversible SAHH inhibitor ameliorates MIA-induced osteoarthritis of rats through suppressing MEK/ERK pathway. Biomed Pharmacother. 2024 Jan

2. Fu Y et al. Effects of H19/SAHH/DNMT1 on the oxidative DNA damage related to benzo[a]pyrene exposure. Environ Sci Pollut Res Int. 2023 Jan

Subcellular Location

Cytoplasm, Melanosome, Nucleus, Endoplasmic reticulum.

UNIPROT

SwissProt: P23526 Human

SwissProt: P50247 Mouse

SwissProt: P10760 Rat

Synonyms

Adenosylhomocysteinase antibody

AdoHcyase antibody

ahcY antibody

S adenosyl L homocysteine hydrolase antibody

S adenosylhomocysteine hydrolase antibody

S-adenosyl-L-homocysteine hydrolase antibody

SAHH antibody

SAHH_HUMAN antibody

Images

  • Western blot analysis of SAHH on different lysates with Rabbit anti-SAHH antibody (<a href="/products/HA722555" style="font-weight: bold;text-decoration: underline;">HA722555</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: Mouse liver tissue lysate<br />Lane 6: Mouse pancreas tissue lysate<br />Lane 7: Rat kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 45 kDa<br /><br />Exposure time: 1 minute 34 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722555" style="font-weight: bold;text-decoration: underline;">HA722555</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SAHH on different lysates with Rabbit anti-SAHH antibody (HA722555) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Mouse liver tissue lysate
    Lane 6: Mouse pancreas tissue lysate
    Lane 7: Rat kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 45 kDa

    Exposure time: 1 minute 34 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722555) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SAHH antibody (<a href="/products/HA722555" style="font-weight: bold;text-decoration: underline;">HA722555</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722555" style="font-weight: bold;text-decoration: underline;">HA722555</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SAHH antibody (HA722555) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-SAHH antibody (<a href="/products/HA722555" style="font-weight: bold;text-decoration: underline;">HA722555</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722555" style="font-weight: bold;text-decoration: underline;">HA722555</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-SAHH antibody (HA722555) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"