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TRAM2 Recombinant Rabbit Monoclonal Antibody [JE41-91]

Usd: 385

Specification

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Catalog# HA722557

TRAM2 Recombinant Rabbit Monoclonal Antibody [JE41-91]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

TRAM2 Recombinant Rabbit Monoclonal Antibody [JE41-91]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human TRAM2 aa 272-287 / 370.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 43 kDa

Positive Control

MG-63 cell lysate, Saos-2 cell lysate, U-2 OS cell lysate, RAW264.7 cell lysate, SP2/0 cell lysate.

Conjugation

unconjugated

Clone Number

JE41-91

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Translocating chain-associated membrane protein 2 is a protein that in humans is encoded by the TRAM2 gene. TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane.

Background References

1. Gao X et al. TRAM2 promotes the malignant progression of glioma through PI3K/AKT/mTOR pathway. Biochem Biophys Res Commun. 2022 Jan

2. Li L et al. A comprehensive enhancer screen identifies TRAM2 as a key and novel mediator of YAP oncogenesis. Genome Biol. 2021 Jan

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q15035 Human

SwissProt: Q924Z5 Mouse

Synonyms

Translocating chain-associated membrane protein 2

TRAM2

KIAA0057

Images

  • Western blot analysis of TRAM2 on different lysates with Rabbit anti-TRAM2 antibody (<a href="/products/HA722557" style="font-weight: bold;text-decoration: underline;">HA722557</a>) at 1/1,000 dilution.<br /><br />Lane 1: MG-63 cell lysate<br />Lane 2: Saos-2 cell lysate<br />Lane 3: U-2 OS cell lysate<br />Lane 4: RAW264.7 cell lysate<br />Lane 5: SP2/0 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722557" style="font-weight: bold;text-decoration: underline;">HA722557</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TRAM2 on different lysates with Rabbit anti-TRAM2 antibody (HA722557) at 1/1,000 dilution.

    Lane 1: MG-63 cell lysate
    Lane 2: Saos-2 cell lysate
    Lane 3: U-2 OS cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: SP2/0 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722557) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"