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Phospho-AMPK alpha 2 (S491) Recombinant Rabbit Monoclonal Antibody [JE59-49]

Usd: 385

Specification

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Catalog# HA722567

Phospho-AMPK alpha 2 (S491) Recombinant Rabbit Monoclonal Antibody [JE59-49]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-AMPK alpha 2 (S491) Recombinant Rabbit Monoclonal Antibody [JE59-49]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser491 of Human AMPK alpha 2.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 62 kDa

Positive Control

HEK-293 cell lysate.

Conjugation

unconjugated

Clone Number

JE59-49

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

5'-AMP-activated protein kinase catalytic subunit alpha-2 is an enzyme that in humans is encoded by the PRKAA2 gene. The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Background References

1. Ni YL et al. Hibifolin protected pro-inflammatory response and oxidative stress in LPS-induced acute lung injury through antioxidative enzymes and the AMPK2/Nrf-2 pathway. Environ Toxicol. 2024 Mar

2. Lin FC et al. Protective Effects of Kirenol against Lipopolysaccharide-Induced Acute Lung Injury through the Modulation of the Proinflammatory NFkappaB Pathway and the AMPK2-/Nrf2-Mediated HO-1/AOE Pathway. Antioxidants (Basel). 2021 Jan

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P54646 Human

Synonyms

5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody

AAPK2_HUMAN antibody

ACACA kinase antibody

Acetyl CoA carboxylase kinase antibody

Acetyl-CoA carboxylase kinase antibody

AMPK alpha 2 chain antibody

AMPK subunit alpha-2 antibody

AMPK2 antibody

AMPKa2 antibody

AMPKalpha2 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-AMPK alpha 2 (S491) on different lysates with Rabbit anti-Phospho-AMPK alpha 2 (S491) antibody (<a href="/products/HA722567" style="font-weight: bold;text-decoration: underline;">HA722567</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HEK-293 cell lysate, the membrane treated with λpp for 1 hour<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 62 kDa<br />Observed band size: 62 kDa<br /><br />Exposure time: 2 minutes 20 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722567" style="font-weight: bold;text-decoration: underline;">HA722567</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-AMPK alpha 2 (S491) on different lysates with Rabbit anti-Phospho-AMPK alpha 2 (S491) antibody (HA722567) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HEK-293 cell lysate, the membrane treated with λpp for 1 hour

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

    Exposure time: 2 minutes 20 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722567) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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