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ADH4 Recombinant Rabbit Monoclonal Antibody [JE62-24]

Usd: 385

Specification

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Catalog# HA722570

ADH4 Recombinant Rabbit Monoclonal Antibody [JE62-24]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ADH4 Recombinant Rabbit Monoclonal Antibody [JE62-24]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human ADH4 aa 331-380 / 380.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 40 kDa

Positive Control

HeLa cell lysate, Human liver tissue lysate, human kidney tissue.

Conjugation

unconjugated

Clone Number

JE62-24

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Alcohol dehydrogenase 4 is an enzyme that in humans is encoded by the ADH4 gene. This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. There is evidence that ancestors of modern humans developed the ADH4 producing gene some 10 million years ago. This probably helped them to take advantage of rotting fruit as they shifted to a terrestrial lifestyle.

Background References

1. Cui J et al. No significant association between SNPs in the CLOCK and ADH4 genes and susceptibility to cluster headaches: A systematic review and meta-analysis. Ann Hum Genet. 2022 Jul

2. Karunakara SH et al. Novel Insights into MEG3/miR664a-3p/ADH4 Axis and Its Possible Role in Hepatocellular Carcinoma from an in Silico Perspective. Genes (Basel). 2022 Nov

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P08319 Human

Synonyms

Ac1002 antibody

ADH 4 antibody

ADH-1 antibody

ADH-2 antibody

ADH2 antibody

Adh4 antibody

ADH4_HUMAN antibody

Alcohol dehydrogenase 4 (class II) pi polypeptide antibody

Alcohol dehydrogenase 4 antibody

Alcohol dehydrogenase class II pi chain antibody

Expand

Images

  • Western blot analysis of ADH4 on different lysates with Rabbit anti-ADH4 antibody (<a href="/products/HA722570" style="font-weight: bold;text-decoration: underline;">HA722570</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: Human liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722570" style="font-weight: bold;text-decoration: underline;">HA722570</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ADH4 on different lysates with Rabbit anti-ADH4 antibody (HA722570) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: Human liver tissue lysate (40 µg/Lane)

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722570) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ADH4 antibody (<a href="/products/HA722570" style="font-weight: bold;text-decoration: underline;">HA722570</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722570" style="font-weight: bold;text-decoration: underline;">HA722570</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ADH4 antibody (HA722570) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"