MRas Recombinant Rabbit Monoclonal Antibody [JE64-57]
Catalog# HA722579
MRas Recombinant Rabbit Monoclonal Antibody [JE64-57]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MRas Recombinant Rabbit Monoclonal Antibody [JE64-57]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human MRas aa 159-208 / 208.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 24 kDa
Positive Control
SH-SY5Y cell lysate, Neuro-2a cell lysate, U-87 MG cell lysate, C6 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-57
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
M-Ras is a member of the small GTPase superfamily under the Ras family, which also includes Rap1, Rap2, R-Ras, and R-Ras2 (TC21). This protein spans a length of 209 residues. Its N-terminal amino acid sequence shares 60-75% identity with that in the Ras protein while its effector region is identical with that in Ras. M-Ras shares a similar structure with H-Ras and Rap2A with the exception of its switch 1 conformation when bound to guanosine 5'-(beta,gamma-imido)triphosphate (Gpp(NH)p). Of the two states M-Ras can switch between, M-Ras is predominantly found in its state 1 conformation, which does not bind Ras effectors.
Background References
1. Young LC et al. MRAS: A Close but Understudied Member of the RAS Family. Cold Spring Harb Perspect Med. 2018 Dec
2. Shah PW et al. MRAS in coronary artery disease-Unchartered territory. IUBMB Life. 2024 Jan
Subcellular Location
Cell membrane.
Synonyms
FLJ42964 antibody
M ras antibody
M-ras antibody
Mras antibody
Muscle and microspikes Ras antibody
Muscle RAS oncogene homolog antibody
Muscle Ras oncogene homologue antibody
Muscle Ras viral oncogene homolog antibody
R ras3 antibody
R-ras3 antibody
ExpandImages
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Western blot analysis of MRas on different lysates with Rabbit anti-MRas antibody (HA722579) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Human brain tissue lysate (40 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 24 kDa
Observed band size: 24 kDa
Exposure time: Lane 1-5: 59 seconds; Lane 6-7: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722579) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"