TNFAIP8 Recombinant Rabbit Monoclonal Antibody [JE65-07]
Catalog# HA722580
TNFAIP8 Recombinant Rabbit Monoclonal Antibody [JE65-07]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TNFAIP8 Recombinant Rabbit Monoclonal Antibody [JE65-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human TNFAIP8 aa 1-50 / 198.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 23 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, A549 cell lysate, THP-1 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate.
Conjugation
unconjugated
Clone Number
JE65-07
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Tumor necrosis factor, alpha-induced protein 8 is a protein that in humans is encoded by the TNFAIP8 gene. It is preferentially expressed in human immune cell types.
Background References
1. Yang F et al. TNFAIP8 overexpression aggravates retinal pathophysiological features of diabetic retinopathy. Exp Eye Res. 2023 Sep
2. Niture S et al. TNFAIP8 drives metabolic reprogramming to promote prostate cancer cell proliferation. Int J Biochem Cell Biol. 2021 Jan
Subcellular Location
Cytoplasm.
Synonyms
GG2 1 antibody
Head and neck tumor and metastasis-related protein antibody
MDC-3.13 antibody
NDED antibody
NF-kappa-B-inducible DED-containing protein antibody
SCC S2 antibody
SCC-S2 antibody
SCCS2 antibody
TFIP8_HUMAN antibody
TNF alpha-induced protein 8 antibody
ExpandImages
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Western blot analysis of TNFAIP8 on different lysates with Rabbit anti-TNFAIP8 antibody (HA722580) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
Lane 6: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 19/21 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"