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Nesprin3 Recombinant Rabbit Monoclonal Antibody [JE65-22]

Usd: 385

Specification

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Catalog# HA722581

Nesprin3 Recombinant Rabbit Monoclonal Antibody [JE65-22]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Nesprin3 Recombinant Rabbit Monoclonal Antibody [JE65-22]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Nesprin3 aa 6776-925 / 975.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 112 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, LNCaP cell lysate.

Conjugation

unconjugated

Clone Number

JE65-22

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Spectrin repeat containing nuclear envelope family member 3, or Nesprin-3, is a Nesprin-family protein that in humans is encoded by the SYNE3 gene. Nesprin-3 localizes to the outer nuclear membrane, where it is retained by SUN domain-containing proteins. The n-terminus of Nesprin-3 faces the cytoplasm and associates with the cytolinker protein Plectin.

Background References

1. Liao L et al. Expression profile of SYNE3 and bioinformatic analysis of its prognostic value and functions in tumors. J Transl Med. 2020 Sep

Subcellular Location

Nucleus outer membrane, Nucleus envelope, Rough endoplasmic reticulum.

UNIPROT

SwissProt: Q6ZMZ3 Human

Synonyms

C14orf49 antibody

Chromosome 14 open reading frame 49 antibody

FLJ25605 antibody

MGC75267 antibody

Nesp3 antibody

Nesprin 3 antibody

Nesprin-3 antibody

NET53 antibody

Nuclear Envelope Spectrin Repeat Protein 3 antibody

Spectrin Repeat Containing, Nuclear Envelope Family Member 3 antibody

Expand

Images

  • Western blot analysis of Nesprin3 on different lysates with Rabbit anti-Nesprin3 antibody (<a href="/products/HA722581" style="font-weight: bold;text-decoration: underline;">HA722581</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: LNCaP cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 112 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722581" style="font-weight: bold;text-decoration: underline;">HA722581</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Nesprin3 on different lysates with Rabbit anti-Nesprin3 antibody (HA722581) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: LNCaP cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 112 kDa
    Observed band size: 100 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722581) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"