Biotin Conjugated Human IL-31 Recombinant Rabbit Monoclonal Antibody [PSH03-02] - Detector
Overview
Product Name
Biotin Conjugated Human IL-31 Recombinant Rabbit Monoclonal Antibody [PSH03-02] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human IL-31 aa 24-164.
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Molecular Weight
Predicted band size: 18.2 kDa
Positive Control
Recombinant Human IL-31 protein (HA210923).
Conjugation
Biotin
Clone Number
PSH03-02
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH03-01] to Human Human IL-31 (Capture) (HA721931) and recombinant Human IL-31 protein (HA210923) as the standard. The reference range value is 46.0-3,800 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein. The mature region shows four alpha -helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region. IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ). In the complex, IL-31 directly binds to GPL, not OSM R. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. Although multiple isoforms of IL-31 RA are known, only a form that contains the
Background References
1. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
2. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
Subcellular Location
Secreted
UNIPROT
Synonyms
IL 31 antibody
IL-31 antibody
IL31 antibody
IL31_HUMAN antibody
Interleukin 31 antibody
Interleukin-31 antibody
Images
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Standard curve of human IL31 matched pair antibodies:
Sandwich ELISA analysis of human IL31 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721931) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted human IL31 protein (HA210923) starting from 3800 pg/ml to 0 pg/ml and detect antibody (HA722631B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of spiked IL-31 in cell culture media samples.
The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"