Biotin Conjugated TNFRSF14/HVEM Recombinant Rabbit Monoclonal Antibody [PSH06-42]
Overview
Product Name
Biotin Conjugated TNFRSF14/HVEM Recombinant Rabbit Monoclonal Antibody [PSH06-42]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human TNFRSF14 aa 39-202.
Species Reactivity
Human
Validated Applications
ELISA(Det)
Positive Control
Recombinant standard Human TNFRSF14 protein (HA210556).
Conjugation
Biotin
Clone Number
PSH06-42
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-41] to Human TNFRSF14 antibody (Capture) (HA722676) and recombinant standard Human TNFRSF14 protein (HA210556) as the standard. The reference range value is 10.3-2,500 pg/mL.
Target
Function
Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network. Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation. Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions. Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response. In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and pro-inflammatory cytokines. Upon binding to CD160 on activated CD4+ T cells, down-regulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response. In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells.
Background References
1. Tamada K., Shimozaki K., Chapoval A.I., Zhai Y., Su J., Chen S.F., Hsieh S.L., Nagata S., Ni J., Chen L. LIGHT, a TNF-like molecule, costimulates T cell proliferation and is required for dendritic cell-mediated allogeneic T cell response. J. Immunol. 164:4105-4110 (2000)
2. Cai G., Anumanthan A., Brown J.A., Greenfield E.A., Zhu B., Freeman G.J. CD160 inhibits activation of human CD4+ T cells through interaction with herpesvirus entry mediator. Nat. Immunol. 9:176-185 (2008)
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
HVEML antibody
ATAR antibody
CD270 antibody
CD40 like protein precursor antibody
Herpes virus entry mediator A antibody
Herpesvirus entry mediator A antibody
Herpesvirus entry mediator antibody
Herpesvirus entry mediator ligand antibody
HveA antibody
HVEM antibody
ExpandImages
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Sandwich ELISA analysis of Human HVEM matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722676) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human TNFRSF14 protein (HA210556) starting from 4,500 pg/ml to 0 pg/ml and detect antibody (HA722678B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native HVEM in human serum samples.
The concentrations of HVEM were measured in triplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean HVEM concentration was determined to be 5,867 pg/ml in human serum. -
Interpolated concentrations of native HVEM in Raji and HL-60 extract samples based on a 1,000 µg/ml extract load.
The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are Raji extract 13% and HL-60 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HVEM concentration was determined to be 3,332 pg/ml in Raji extract and 1,901 pg/ml in HL-60 extract. -
Interpolated concentrations of spiked HVEM in human cell culture media samples.
The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TNFRSF14/HVEM Recombinant Rabbit Monoclonal Antibody [PSH06-41] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
