Biotin Conjugated Human Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH06-46]
Overview
Product Name
Biotin Conjugated Human Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH06-46]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Complement C5 19-821.
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant standard Human Complement C5 protein (HA210636).
Conjugation
Biotin
Clone Number
PSH06-46
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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ELISA(Det)
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Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-45] to Human Complement C5 antibody (Capture) (HA722682) and recombinant standard Human Complement C5 protein (HA210636) as the standard. The reference range value is 111-9,000 pg/mL.
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ELISA
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Use at an assay dependent concentration.
Target
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
Background References
1. DeMartino J.A., Van Riper G., Siciliano S.J., Molineaux C.J., Konteatis Z.D., Rosen H., Springer M.S. The amino terminus of the human C5a receptor is required for high affinity C5a binding and for receptor activation by C5a but not C5a analogs. J. Biol. Chem. 269:14446-14450 (1994)
2. Hillebrandt S., Wasmuth H.E., Weiskirchen R., Hellerbrand C., Keppeler H., Werth A., Schirin-Sokhan R., Wilkens G., Geier A., Lammert F. Complement factor 5 is a quantitative trait gene that modifies liver fibrogenesis in mice and humans. Nat. Genet. 37:835-843 (2005)
Subcellular Location
Secreted.
UNIPROT
Synonyms
Anaphylatoxin C5a analog antibody
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
C5 antibody
C5a anaphylatoxin antibody
C5a antibody
C5b antibody
CO5_HUMAN antibody
Complement C5 alpha'' chain antibody
Complement C5 antibody
Complement component C5 antibody
ExpandImages
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Sandwich ELISA analysis of human Complement C5 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722682) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human Complement C5 protein starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722684B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentration of native Complement C5 was measured in duplicate at different sample concentrations. Undiluted samples were 1:10000 serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Complement C5 concentration was determined to be 78.3 ug/mL in human serum.
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Interpolated concentrations of native Complement C5 in human cell culture supernatant samples.
Complement C5 was measured in 100% cell supernatant. The concentrations of Complement C5 were interpolated from the Complement C5 standard curves. The mean Complement C5 concentration was determined to be 5.256 ng/mL in HepG2 cell supernanant. There was no detectable signal in U-2 OS cell supernatant. -
Interpolated concentrations of spiked Complement C5 in human cell culture media samples.
The concentrations of Complement C5 were measured in duplicates, interpolated from the Complement C5 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"