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Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]

Usd: 385

Specification

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Catalog# HA722686

Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Torsin A / DYT1 aa 283-332 / 332.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 38 kDa

Positive Control

HepG2 cell lysate, 293T cell lysate, A549 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE01-51

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Torsin-1A (TorA) also known as dystonia 1 protein (DYT1) is a protein that in humans is encoded by the TOR1A gene (also known as DQ2 or DYT1). TorA localizes to the endoplasmic reticulum and contiguous perinuclear space, where its ATPase activity is activated by either LULL1 or LAP1, respectively. The protein encoded by this gene is a member of the AAA family of adenosine triphosphatases (ATPases), is related to the Clp protease/heat shock family.

Background References

1. Fan Y et al. DYT-TOR1A dystonia: an update on pathogenesis and treatment. Front Neurosci. 2023 Aug

2. Pocratsky AM et al. Pathophysiology of Dyt1-Tor1a dystonia in mice is mediated by spinal neural circuit dysfunction. Sci Transl Med. 2023 May

Subcellular Location

Endoplasmic reticulum lumen, Nucleus membrane, Cell projection, growth cone, Cytoplasmic vesicle membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle, Cytoplasm, cytoskeleton.

UNIPROT

SwissProt: O14656 Human

Synonyms

DQ2 antibody

Dystonia 1 antibody

Dystonia 1 protein antibody

Dystonia 1 torsion antibody

Dyt1 antibody

TOR1A antibody

TOR1A_HUMAN antibody

Torsin 1A antibody

Torsin A antibody

Torsin family 1 member A antibody

Expand

Images

  • Western blot analysis of Torsin A / DYT1 on different lysates with Rabbit anti-Torsin A / DYT1 antibody (<a href="/products/HA722686" style="font-weight: bold;text-decoration: underline;">HA722686</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate (20 µg/Lane)<br />Lane 2: 293T cell lysate (20 µg/Lane)<br />Lane 3: A549 cell lysate (20 µg/Lane)<br />Lane 4: SW480 cell lysate (20 µg/Lane)<br />Lane 5: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 6: C6 cell lysate (20 µg/Lane)<br />Lane 7: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 35 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722686" style="font-weight: bold;text-decoration: underline;">HA722686</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Torsin A / DYT1 on different lysates with Rabbit anti-Torsin A / DYT1 antibody (HA722686) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate (20 µg/Lane)
    Lane 2: 293T cell lysate (20 µg/Lane)
    Lane 3: A549 cell lysate (20 µg/Lane)
    Lane 4: SW480 cell lysate (20 µg/Lane)
    Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 6: C6 cell lysate (20 µg/Lane)
    Lane 7: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 35 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722686) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"