CMT2 Recombinant Rabbit Monoclonal Antibody [JE06-60]
Catalog# HA722690
CMT2 Recombinant Rabbit Monoclonal Antibody [JE06-60]
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WB
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Human
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unconjugated
Overview
Product Name
CMT2 Recombinant Rabbit Monoclonal Antibody [JE06-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human CMT2 aa 225-274 / 274.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 31 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
JE06-60
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
MAD2L1-binding protein is a protein that in humans is encoded by the MAD2L1BP gene. The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Background References
1. Huang L et al. Biallelic variants in MAD2L1BP (p31(comet)) cause female infertility characterized by oocyte maturation arrest. Elife. 2023 Jun
2. Abdel-Salam GMH et al. Biallelic MAD2L1BP (p31comet) mutation is associated with mosaic aneuploidy and juvenile granulosa cell tumors. JCI Insight. 2023 Nov
Subcellular Location
Nucleus, Cytoplasm, cytoskeleton, spindle.
UNIPROT
Synonyms
Caught by MAD2 protein antibody
CMT2 antibody
dJ261G23.1 antibody
KIAA0110 antibody
MAD2L1 binding protein antibody
MAD2L1 binding protein isoform 2 antibody
MAD2L1-binding protein antibody
Mad2l1bp antibody
MD2BP_HUMAN antibody
MGC11282 antibody
ExpandImages
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Western blot analysis of CMT2 on different lysates with Rabbit anti-CMT2 antibody (HA722690) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A431 cell lysate
Lane 4: A549 cell lysate
Lane 5: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722690) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"