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AMPK gamma 1 Recombinant Rabbit Monoclonal Antibody [JE07-47]

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Specification

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Catalog# HA722691

AMPK gamma 1 Recombinant Rabbit Monoclonal Antibody [JE07-47]

Application

  • WB

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

AMPK gamma 1 Recombinant Rabbit Monoclonal Antibody [JE07-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human AMPK gamma 1 aa 282-331 / 331.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 38 kDa

Positive Control

HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate.

Conjugation

unconjugated

Clone Number

JE07-47

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IP

  • 1-2μg/sample

Target

Function

5'-AMP-activated protein kinase subunit gamma-1 is an enzyme that in humans is encoded by the PRKAG1 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Background References

1. Ripa R et al. Refeeding-associated AMPKγ1 complex activity is a hallmark of health and longevity. Nat Aging. 2023 Dec

Subcellular Location

Cytoplasm, cytosol, membrane, nucleoplasm, nucleus.

UNIPROT

SwissProt: P54619 Human

SwissProt: O54950 Mouse

SwissProt: P80385 Rat

Synonyms

5' AMP activated protein kinase gamma 1 subunit antibody

5' AMP activated protein kinase subunit gamma 1 antibody

5''-AMP-activated protein kinase subunit gamma-1 antibody

AAKG1_HUMAN antibody

AMP activated protein kinase noncatalytic gamma 1 subunit antibody

AMPK gamma 1 chain antibody

AMPK gamma1 antibody

AMPK subunit gamma-1 antibody

AMPKg antibody

MGC8666 antibody

Expand

Images

  • Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (<a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HEK-293 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: Jurkat cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 3 minutes; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HEK-293 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Jurkat cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 3 minutes; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (<a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: Mouse heart tissue lysate<br />Lane 3: Rat heart tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: Mouse heart tissue lysate
    Lane 3: Rat heart tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • AMPK gamma 1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with <a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: Jurkat cell lysate (input)<br />Lane 2: <a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a> IP in Jurkat cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722691" style="font-weight: bold;text-decoration: underline;">HA722691</a> in Jurkat cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    AMPK gamma 1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722691 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722691 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: Jurkat cell lysate (input)
    Lane 2: HA722691 IP in Jurkat cell lysate
    Lane 3: Rabbit IgG instead of HA722691 in Jurkat cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation