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HES5 Recombinant Rabbit Monoclonal Antibody [JE35-96]

Usd: 385

Specification

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Catalog# HA722699

HES5 Recombinant Rabbit Monoclonal Antibody [JE35-96]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

HES5 Recombinant Rabbit Monoclonal Antibody [JE35-96]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human HES5 aa 1-166 / 166.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 18 kDa

Positive Control

HEK-293 cell lysate, U-87 MG cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate, human glioblastoma tissue, human small intestine tissue.

Conjugation

unconjugated

Clone Number

JE35-96

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Transcription factor HES-5 is a protein that in humans is encoded by the HES5 gene. HES5 regulates the development of the early brain by maintaining stem cell neural progenitors in the ventricular zone. HES5 expression significantly higher in squamous cervical carcinoma than in CIN as well as higher in CIN than normal cervical epithelia. Human HES5 gene binds to Notch receptor and expression of HES5 decreases during cartilage differentiation.

Background References

1. Morris HE et al. Notch3/Hes5 Induces Vascular Dysfunction in Hypoxia-Induced Pulmonary Hypertension Through ER Stress and Redox-Sensitive Pathways. Hypertension. 2023 Aug

2. Miao X et al. HES5-mediated repression of LIGHT transcription may contribute to apoptosis in hepatocytes. Cell Death Discov. 2021 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q5TA89 Human

SwissProt: P70120 Mouse

SwissProt: Q03062 Rat

Synonyms

bHLHb38 antibody

Class B basic helix-loop-helix protein 38 antibody

Hairy and enhancer of split 5 antibody

Hairy/Enhancer of spli, Drosophila, homolog of, 5 antibody

HES 5 antibody

hes family bHLH transcription factor 5 antibody

Hes5 antibody

HES5 protein antibody

HES5_HUMAN antibody

MGI:104876 antibody

Expand

Images

  • Western blot analysis of HES5 on different lysates with Rabbit anti-HES5 antibody (<a href="/products/HA722699" style="font-weight: bold;text-decoration: underline;">HA722699</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: U-87 MG cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: Mouse kidney tissue lysate<br />Lane 5: Rat liver tissue lysate<br />Lane 6: Rat kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 18 kDa<br />Observed band size: 18 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722699" style="font-weight: bold;text-decoration: underline;">HA722699</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HES5 on different lysates with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: U-87 MG cell lysate
    Lane 3: C6 cell lysate
    Lane 4: Mouse kidney tissue lysate
    Lane 5: Rat liver tissue lysate
    Lane 6: Rat kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722699) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue with Rabbit anti-HES5 antibody (<a href="/products/HA722699" style="font-weight: bold;text-decoration: underline;">HA722699</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722699" style="font-weight: bold;text-decoration: underline;">HA722699</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722699) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HES5 antibody (<a href="/products/HA722699" style="font-weight: bold;text-decoration: underline;">HA722699</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722699" style="font-weight: bold;text-decoration: underline;">HA722699</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722699) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"