Complement factor B Recombinant Rabbit Monoclonal Antibody [JE54-96]
Catalog# HA722704
Complement factor B Recombinant Rabbit Monoclonal Antibody [JE54-96]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Complement factor B Recombinant Rabbit Monoclonal Antibody [JE54-96]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Complement factor B aa 715-764 / 764.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 86 kDa
Positive Control
Human lung tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate.
Conjugation
unconjugated
Clone Number
JE54-96
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease that associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC class III) region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.
Background References
1. Peng M et al. Complement factor B/C2 in molluscs regulates agglutination and illuminates evolution of the Bf/C2 family. FASEB J. 2019 Dec
2. Akhlaghpour M et al. Genetic coding variant in complement factor B (CFB) is associated with increased risk for perianal Crohn\'s disease and leads to impaired CFB cleavage and phagocytosis. Gut. 2023 Nov
Subcellular Location
Secreted.
Synonyms
C3/C5 convertase antibody
Complement factor B antibody
Glycine-rich beta glycoprotein antibody
PBF2 antibody
Properdin factor B antibody
Images
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Western blot analysis of Complement factor B on different lysates with Rabbit anti-Complement factor B antibody (HA722704) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Mouse lung tissue lysate
Lane 3: Rat lung tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 86 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722704) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"