DCTN5 Recombinant Rabbit Monoclonal Antibody [JE58-30]
Catalog# HA722706
DCTN5 Recombinant Rabbit Monoclonal Antibody [JE58-30]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
DCTN5 Recombinant Rabbit Monoclonal Antibody [JE58-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human DCTN5 aa 1-182 / 182.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 20 kDa
Positive Control
MCF7 cell lysate, 293T cell lysate, K-562 cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, human breast cancer tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
Clone Number
JE58-30
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:200
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IP
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1-2μg/sample
Target
Function
Dynactin 5 (p25) is a protein that in humans is encoded by the DCTN5 gene. This gene encodes a subunit of dynactin, a component of the cytoplasmic dynein motor machinery involved in minus-end-directed transport. The encoded protein is a component of the pointed-end subcomplex and is thought to bind membranous cargo. A pseudogene of this gene is located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Background References
1. Wei M et al. Cloning, characterization and functional analysis of dctn5 in immune response of Chinese tongue sole (Cynoglossus semilaevis). Fish Shellfish Immunol. 2018 Jun
Subcellular Location
Cytoplasm, cytoskeleton, Chromosome, centromere, kinetochore.
Synonyms
DCTN 5 antibody
DCTN5 antibody
DCTN5_HUMAN antibody
Dynactin 4 antibody
Dynactin 5 (p25) antibody
Dynactin subunit 5 antibody
Dynactin subunit p25 antibody
p25 antibody
Images
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Western blot analysis of DCTN5 on different lysates with Rabbit anti-DCTN5 antibody (HA722706) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (20 µg/Lane)
Lane 6: PC-12 cell lysate (20 µg/Lane)
Lane 7: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722706) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-DCTN5 antibody (HA722706) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722706) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-DCTN5 antibody (HA722706) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722706) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-DCTN5 antibody (HA722706) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722706) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
DCTN5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722706 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722706 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF7 cell lysate (input)
Lane 2: HA722706 IP in MCF7 cell lysate
Lane 3: Rabbit IgG instead of HA722706 in MCF7 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"