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CRX Recombinant Rabbit Monoclonal Antibody [JE60-71]

Usd: 385

Specification

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Catalog# HA722707

CRX Recombinant Rabbit Monoclonal Antibody [JE60-71]

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CRX Recombinant Rabbit Monoclonal Antibody [JE60-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within mouse CRX aa 250-299 / 299.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 32 kDa

Positive Control

Mouse eyeball tissue lysate, Rat eyeball tissue lysate, mouse eye tissue, rat eye tissue.

Conjugation

unconjugated

Clone Number

JE60-71

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Cone-rod homeobox protein is a protein that in humans is encoded by the CRX gene. The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber's congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined.

Background References

1. Sun C et al. Gene Augmentation for Autosomal Dominant CRX-Associated Retinopathies. Adv Exp Med Biol. 2023

2. Pan D et al. CRX haploinsufficiency compromises photoreceptor precursor translocation and differentiation in human retinal organoids. Stem Cell Res Ther. 2023 Dec

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O54751 Mouse

Entrez Gene: 60446 Rat

Synonyms

Cone rod homeobox antibody

Cone rod homeobox protein antibody

Cone rod homeobox-containing gene antibody

cone-rod homeobox antibody

Cone-rod homeobox protein antibody

CORD 2 antibody

CRD antibody

CRX antibody

CRX_HUMAN antibody

LCA 7 antibody

Expand

Images

  • Western blot analysis of CRX on different lysates with Rabbit anti-CRX antibody (<a href="/products/HA722707" style="font-weight: bold;text-decoration: underline;">HA722707</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse eyeball tissue lysate<br />Lane 2: Rat eyeball tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 32 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: Lane 1: 46 seconds; Lane 2: 3 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722707" style="font-weight: bold;text-decoration: underline;">HA722707</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CRX on different lysates with Rabbit anti-CRX antibody (HA722707) at 1/1,000 dilution.

    Lane 1: Mouse eyeball tissue lysate
    Lane 2: Rat eyeball tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 32 kDa
    Observed band size: 40 kDa

    Exposure time: Lane 1: 46 seconds; Lane 2: 3 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722707) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse eye tissue with Rabbit anti-CRX antibody (<a href="/products/HA722707" style="font-weight: bold;text-decoration: underline;">HA722707</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722707" style="font-weight: bold;text-decoration: underline;">HA722707</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue with Rabbit anti-CRX antibody (HA722707) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722707) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat eye tissue with Rabbit anti-CRX antibody (<a href="/products/HA722707" style="font-weight: bold;text-decoration: underline;">HA722707</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722707" style="font-weight: bold;text-decoration: underline;">HA722707</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat eye tissue with Rabbit anti-CRX antibody (HA722707) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"