AZI2 Recombinant Rabbit Monoclonal Antibody [JE64-98]
Catalog# HA722711
AZI2 Recombinant Rabbit Monoclonal Antibody [JE64-98]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
AZI2 Recombinant Rabbit Monoclonal Antibody [JE64-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human AZI2 aa 243-392 / 392.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, K-562 cell lysate, LNCaP cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE64-98
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
5-azacytidine-induced protein 2 is a protein that in humans is encoded by the AZI2 gene. AZI2, or NAP1, contributes to the activation of NFKB -dependent gene expression by activating IKK-related kinases, such as NAK.
Background References
1. Yeo SK et al. AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer. Autophagy. 2024 Mar
2. Wei M et al. AZI2 positively regulates the induction of type I interferon in influenza-trigger pediatric pneumonia. Pathog Dis. 2022 Jul
Subcellular Location
Cytoplasm.
Synonyms
5-azacytidine-induced protein 2
NF-kappa-B-activating kinase-associated protein 1
Nak-associated protein 1
Nap1
TILP
AZI2
NAP1
TBKBP2
Images
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Western blot analysis of AZI2 on different lysates with Rabbit anti-AZI2 antibody (HA722711) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: LNCaP cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 48 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722711) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"